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Sample GSM149674 Query DataSets for GSM149674
Status Public on Nov 08, 2007
Title Dyak_male_rep4
Sample type RNA
 
Source name Drosophila yakuba adult male
Organism Drosophila yakuba
Characteristics 5-7 day aged adult flies
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using Trizol procedure, followed by mRNA isolation using an Oligotex mRNA Kit (Qiagen, Valencia, CA).
Label Cy3 or Cy5
Label protocol 600ng adult fly poly(A)+ RNA was labeled with Cy3 or Cy5 random nonamer primer oligonucleotides (TriLink Biotechnologies, San Diego, CA) by reversed transcription in the presence of SuperScript II Reverse Transcriptase (Invitrogen). The labeled sample was purified with Chroma Spin column TE-10 (Clontech, Mountain View, CA).
 
Hybridization protocol CPK6 50mer fiducial oligo control and hybridization buffer were added, and samples were applied to microarrays enclosed in custome-made chambers at 42C for 16-20 hours. Slides were then washed according to the protocol described by Nimblegen (Madison, WI) and dried by centrifugation.
Scan protocol Scanned on an Axon GenePix 4000B scanner. Data were extracted from the scanned images using the NimbleScan 2.1 program (NimbleGen Systems, Inc.).
Description Biological replicate 4 of 7.
Data processing VSN normalized.
 
Submission date Dec 11, 2006
Last update date Apr 25, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL4634
Series (1)
GSE6640 Profiling of sex-biased expression in Drosophila species

Data table header descriptions
ID_REF NimbleGen PROBE_DESIGN_ID
VALUE Log2 transformed signal intensities after VSN normalization
Raw_Intensity raw intensities

Data table
ID_REF VALUE Raw_Intensity
2214_0001_0001 5.722 90.33
2214_0004_0001 14.909 17696.33
2214_0006_0001 14.944 18140.56
2214_0008_0001 14.868 17211
2214_0010_0001 14.722 15557.56
2214_0012_0001 14.89 17467.33
2214_0014_0001 15.035 19314.89
2214_0016_0001 15.025 19183.56
2214_0018_0001 14.833 16797.44
2214_0020_0001 14.71 15432.33
2214_0022_0001 14.819 16640.67
2214_0025_0001 8.827 332.33
2214_0027_0001 10.27 780.56
2214_0029_0001 9.755 567.78
2214_0031_0001 9.401 460
2214_0033_0001 13.644 7407.78
2214_0035_0001 8.977 361
2214_0037_0001 7.233 155.11
2214_0039_0001 6.588 121.33
2214_0041_0001 7.295 159.11

Total number of rows: 391015

Table truncated, full table size 10684 Kbytes.




Supplementary file Size Download File type/resource
GSM149674.ftr.gz 8.3 Mb (ftp)(http) FTR
Processed data included within Sample table

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