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Sample GSM149681 Query DataSets for GSM149681
Status Public on Nov 08, 2007
Title Dana_female_rep3
Sample type RNA
 
Source name Drosophila ananassae adult female
Organism Drosophila ananassae
Characteristics 5-7 day aged adult flies
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using Trizol procedure, followed by mRNA isolation using an Oligotex mRNA Kit (Qiagen, Valencia, CA).
Label Cy3 or Cy5
Label protocol 600ng adult fly poly(A)+ RNA was labeled with Cy3 or Cy5 random nonamer primer oligonucleotides (TriLink Biotechnologies, San Diego, CA) by reversed transcription in the presence of SuperScript II Reverse Transcriptase (Invitrogen). The labeled sample was purified with Chroma Spin column TE-10 (Clontech, Mountain View, CA).
 
Hybridization protocol CPK6 50mer fiducial oligo control and hybridization buffer were added, and samples were applied to microarrays enclosed in custome-made chambers at 42C for 16-20 hours. Slides were then washed according to the protocol described by Nimblegen (Madison, WI) and dried by centrifugation.
Scan protocol Scanned on an Axon GenePix 4000B scanner. Data were extracted from the scanned images using the NimbleScan 2.1 program (NimbleGen Systems, Inc.).
Description Biological replicate 3 of 6.
Data processing VSN normalized.
 
Submission date Dec 11, 2006
Last update date Apr 25, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL4628
Series (1)
GSE6640 Profiling of sex-biased expression in Drosophila species

Data table header descriptions
ID_REF NimbleGen PROBE_DESIGN_ID
VALUE Log2 transformed signal intensities after VSN normalization
Raw_Intensity raw intensities

Data table
ID_REF VALUE Raw_Intensity
2209_0001_0001 9.192 439.56
2209_0004_0001 15.854 37200.22
2209_0006_0001 15.725 34003.78
2209_0008_0001 15.831 36596.67
2209_0010_0001 15.926 39091.56
2209_0012_0001 15.984 40693.56
2209_0014_0001 15.905 38517.89
2209_0016_0001 15.867 37529.78
2209_0018_0001 15.905 38530.44
2209_0020_0001 15.8 35820.89
2209_0022_0001 15.893 38218.56
2209_0025_0001 6.786 134.67
2209_0027_0001 7.22 161.33
2209_0029_0001 9.417 501.67
2209_0031_0001 8.332 273.44
2209_0033_0001 7.153 156.78
2209_0035_0001 8.627 319.89
2209_0037_0001 8.452 291.22
2209_0039_0001 8.606 316.33
2209_0041_0001 12.315 3268.56

Total number of rows: 391015

Table truncated, full table size 10691 Kbytes.




Supplementary file Size Download File type/resource
GSM149681.ftr.gz 8.3 Mb (ftp)(http) FTR
Processed data included within Sample table

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