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Sample GSM149834 Query DataSets for GSM149834
Status Public on Mar 01, 2007
Title hy5 mutant vs wildtype expression, rep3
Sample type RNA
 
Channel 1
Source name hy5 mutant
Organism Arabidopsis thaliana
Characteristics 4 days hy5 mutant seedling
Growth protocol continuous white light for 4 days
Extracted molecule total RNA
Extraction protocol Trizol/RNeasy
Label Cy5
Label protocol amino-allyl and monofunctional dye
 
Channel 2
Source name wildtype reference
Organism Arabidopsis thaliana
Characteristics 4 days wildtype seedling
Extracted molecule total RNA
Extraction protocol Trizol/RNeasy
Label Cy3
Label protocol amino-allyl and monofunctional dye
 
 
Hybridization protocol 42C 50% formamide
Scan protocol Axon GenePix 4000B
Description hy5 mutant vs. wildtype
Data processing LIMMA in bioconductor R package
 
Submission date Dec 13, 2006
Last update date Dec 13, 2006
Contact name Kun He
E-mail(s) hek@mail.cbi.pku.edu.cn
Phone 203-432-8909
Organization name Yale University
Department Molecular, Cellular & Developmental Biology
Lab Denglab
Street address 165 Prospect
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platform ID GPL988
Series (1)
GSE6517 expression profiling of wild type and the hy5 mutant in arabidopsis

Data table header descriptions
ID_REF
VALUE normalized log ratio of CH1/CH2
CH1_SIG_MEAN Channel 1 (Cy5, hy5 mutant) mean pixel intensity
CH1_BKG_MEDIAN Channel 1 Background median pixel intensity
CH2_SIG_MEAN Channel 2 (Cy3, wildtype) mean pixel intensity
CH2_BKG_MEDIAN Channel 2 Background median pixel intensity

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKG_MEDIAN CH2_SIG_MEAN CH2_BKG_MEDIAN
3203159 null 502 442 475 488
3203161 0.132367340791671 1827 434 2015 497
3203163 null 465 459 481 492
3203165 1.02654455588907 536 448 534 478
3203167 null 471 439 470 483
3203169 0.872461124940675 554 436 551 470
3203171 null 476 420 431 478
3203173 -0.55591044637839 560 457 665 481
3203175 null 446 449 468 481
3203177 0.273149272112134 666 479 673 469
3203179 0.189586751831296 724 454 808 467
3203181 1.15794145187200 552 439 539 474
3203183 0.788009827648413 489 437 490 469
3203185 null 439 419 461 464
3203187 null 527 434 464 467
3203189 -1.20636779401968 463 441 478 463
3203191 null 453 443 455 472
3203193 -0.888966478741168 484 457 489 460
3203195 -0.163125744964208 631 497 671 486
3203197 -0.308418083445968 579 455 680 492

Total number of rows: 27648

Table truncated, full table size 1129 Kbytes.




Supplementary data files not provided

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