|
Status |
Public on Dec 18, 2006 |
Title |
gh3.5-1D 48 hpi, biological replicate 1 |
Sample type |
RNA |
|
|
Source name |
Arabidopsis thaliana mutant gh3.5-1D leaves, 48 hpi with Pst DC3000(avrRpt2)
|
Organism |
Arabidopsis thaliana |
Characteristics |
Genotype: gh3.5-1D, mutant Age: 5 week-old Tissue: 48 hpi with Pst DC3000(avrRpt2) leaves
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from the leaves using the Trizol method (Invitrogen) and purified with RNeasy MiniKit (Qiagen). cDNA was synthesized and purified with one-cycle cDNA Synthesis Kit (Affymetrix) and GeneChip Sample Cleanup Module (Affymetrix), respectively.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol with GeneChip IVT Labeling Kit and Genechip Sample Cleanup Module (Affymetrix)
|
|
|
Hybridization protocol |
Affymetrix standard protocol
|
Scan protocol |
Affymetrix standard protocol
|
Description |
Gene expression data of mutant gh3.5-1D leaves after infection with Pst DC3000(avrRpt2)
|
Data processing |
The data were analyzed with Affymetrix GeneChip® Operating Software (GCOS, Version 1.4) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
|
|
Submission date |
Dec 18, 2006 |
Last update date |
Aug 28, 2018 |
Contact name |
Li Qun |
E-mail(s) |
qli@sibs.ac.cn
|
Phone |
+86 21 54924122
|
Organization name |
Institute of plant physiology & ecology, SIBS, CAS
|
Department |
National Key Laboratory of Plant Molecular Genetics
|
Street address |
300 Fenglin Road
|
City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
|
|
Platform ID |
GPL198 |
Series (1) |
GSE6556 |
Expression profiling of A. thaliana wild type Columbia-0 and mutant gh3.5-1D in response to pathogen Pst DC3000(avrRpt2) |
|
Relations |
Reanalyzed by |
GSE118579 |
Reanalyzed by |
GSE119083 |