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Sample GSM151705 Query DataSets for GSM151705
Status Public on Dec 18, 2006
Title gh3.5-1D 48 hpi, biological replicate 3
Sample type RNA
 
Source name Arabidopsis thaliana mutant gh3.5-1D leaves, 48 hpi with Pst DC3000(avrRpt2)
Organism Arabidopsis thaliana
Characteristics Genotype: gh3.5-1D, mutant Age: 5 week-old Tissue: 48 hpi with Pst DC3000(avrRpt2) leaves
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the leaves using the Trizol method (Invitrogen) and purified with RNeasy MiniKit (Qiagen). cDNA was synthesized and purified with one-cycle cDNA Synthesis Kit (Affymetrix) and GeneChip Sample Cleanup Module (Affymetrix), respectively.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol with GeneChip IVT Labeling Kit and Genechip Sample Cleanup Module (Affymetrix)
 
Hybridization protocol Affymetrix standard protocol
Scan protocol Affymetrix standard protocol
Description Gene expression data of mutant gh3.5-1D leaves after infection with Pst DC3000(avrRpt2)
Data processing The data were analyzed with Affymetrix GeneChip® Operating Software (GCOS, Version 1.4) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Dec 18, 2006
Last update date Aug 28, 2018
Contact name Li Qun
E-mail(s) qli@sibs.ac.cn
Phone +86 21 54924122
Organization name Institute of plant physiology & ecology, SIBS, CAS
Department National Key Laboratory of Plant Molecular Genetics
Street address 300 Fenglin Road
City Shanghai
State/province Shanghai
ZIP/Postal code 200032
Country China
 
Platform ID GPL198
Series (1)
GSE6556 Expression profiling of A. thaliana wild type Columbia-0 and mutant gh3.5-1D in response to pathogen Pst DC3000(avrRpt2)
Relations
Reanalyzed by GSE118579
Reanalyzed by GSE119083

Data table header descriptions
ID_REF Affymetrix Probe Set ID
VALUE Affymetrix GeneChip Operating Software (GCOS, Version 1.4, Affymetrix) calculated Signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
244901_at 155.2 P 0.000244
244902_at 53.2 P 0.000244
244903_at 102 P 0.000732
244904_at 23.5 P 0.030273
244905_at 8.7 A 0.165771
244906_at 87.6 P 0.001221
244907_at 4.9 A 0.246094
244908_at 0.5 A 0.989258
244909_at 3.2 A 0.567627
244910_s_at 16.3 P 0.023926
244911_at 1.1 A 0.696289
244912_at 58.4 P 0.00293
244913_at 0.9 A 0.976074
244914_at 0.8 A 0.828613
244915_s_at 4.8 A 0.567627
244916_at 8.8 A 0.219482
244917_at 1.5 A 0.953857
244918_at 1.3 A 0.828613
244919_at 20 P 0.005859
244920_s_at 25.2 P 0.010742

Total number of rows: 22810

Table truncated, full table size 573 Kbytes.




Supplementary file Size Download File type/resource
GSM151705.CEL.gz 3.2 Mb (ftp)(http) CEL

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