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Sample GSM1521891 Query DataSets for GSM1521891
Status Public on Oct 10, 2014
Title MLL-AF9-GFP_3μM_UNC2400_D5_5
Sample type RNA
 
Source name MLL-AF9-GFP_3μM_UNC2400_D5
Organism Mus musculus
Characteristics strain background: Balb/c
cell line: MLL-AF9-IRES-GFP
cell type: transformed murine leukemia progenitor cell line
treated with: UNC2400 (inactive analog of UNC1999)
Treatment protocol Treatment with 3μM of the indicated compounds for 4-5 days, or induction of gene-specific shRNAs (with an inducible TRMPVIR system) for 6 days, followed by total RNA preparation for microarray.
Growth protocol Transformed murine leukemia progenitor cell lines were cultured in Opti-MEM base medium supplemented with 10% FBS, 1% antibiotics, 50 μM mercaptoethanol and stem cell factor
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with the Rneasy Plus Miniprep Kit (Qiagen).
Label Biotin
Label protocol The cDNA was fragmented and terminally labeled with biotin, using the Affymetrix GeneChip WT Terminal Labeling kit (Affymetrix, Santa Clara, CA)
 
Hybridization protocol Samples were hybridized with Affymetrix Mouse Gene 2.1 ST Array Strip (Affymetrix) according to the manifacture's instruction.
Scan protocol Standard Affymetrix protocol
Description MLL-AF9-GFP_3uM_UNC2400_D5_5
Data processing Hybridization signals were retrieved and normalized, followed by differential expression analysis and statistical analysis using GeneSpring Analysis Platform GX 12.6 (Agilent Technologies).
probe group file: MoGene-2_1-st.pgf
meta-probeset file: MoGene-2_1-st.mps
 
Submission date Oct 09, 2014
Last update date Oct 10, 2014
Contact name Bowen Xu
E-mail(s) bowenx@email.unc.edu
Phone 919-966-5953
Organization name UNC-Chapel Hill
Department Biochemistry and Biophysics
Lab Wang
Street address 450 West Drive, CB7295, Lineberger Cancer Center, GREG WANG Lab, RM 31-331
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platform ID GPL17400
Series (1)
GSE62198 Expression data of MLL-AF9-transformed murine leukemia progenitor cells, following treatment with UNC1999, a dual Ezh1 and Ezh2 inhibitor, or knockdown of EED

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
17200001 226.8475
17200003 283.7733
17200005 133.7309
17200007 84.02954
17200009 108.625
17200011 178.0372
17200013 71.29612
17200015 72.80322
17200017 28.59107
17200019 25.19196
17200021 67.51807
17200023 144.4333
17200025 139.3263
17200027 123.3876
17200029 82.89925
17200031 69.85275
17200033 47.44186
17200035 33.31635
17200037 75.79476
17200039 31.02235

Total number of rows: 41345

Table truncated, full table size 722 Kbytes.




Supplementary file Size Download File type/resource
GSM1521891_MLL-AF9-GFP_3__M_UNC2400_D5_5.CEL.gz 8.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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