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| Status |
Public on Oct 10, 2014 |
| Title |
Expression data of MLL-AF9-transformed murine leukemia progenitor cells, following treatment with UNC1999, a dual Ezh1 and Ezh2 inhibitor, or knockdown of EED |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Ezh2 and EZH1 are histone H3 lysine 27 specific methyltransferase. Their hyperactive mutations and overexpression were found in cancer including various hematological malignancies. UNC1999 is a highly selective inhibitor for both enzymes. It suppresses H3K27 tri- and di-methylation globally and inhibits growth of MLL-rearranged acute leukemia cell lines. UNC2400, a di-methylated derivative of UNC1999, is employed an inactive analog compound for assessment of off-target effects. EED knockdown was used to demonstrate gene targets of PRC2.
Here we performed microarray analysis in MLL-AF9 transformed acute leukemia cells following treatment with DMSO, UNC2400 or UNC1999, or after induction of EED shRNA (Renilla shRNA as control). This study allows us to identify UNC1999-responsive gene signatures as well as their overlap with PRC2 targets in MLL-AF9-bearing leukemia cells.
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| Overall design |
We analyzed 5 of DMSO-treated, 5 of UNC2400 (inactive analog of UN1999)-treated, and 6 of UNC1999-treated MLL-AF9 leukemia cell samples to identify UNC1999-responsive gene signatures. We also performed microarray in 3 of shRNA control lines (Renilla or Ren) and 3 of shEED lines to identify downstream targets of PRC2 complex in MLL-AF9 leukemia cells.
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| Contributor(s) |
Xu B, ON D, Wang GG |
| Citation(s) |
25395428 |
| |
| Submission date |
Oct 09, 2014 |
| Last update date |
Mar 08, 2018 |
| Contact name |
Bowen Xu |
| E-mail(s) |
bowenx@email.unc.edu
|
| Phone |
919-966-5953
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Biochemistry and Biophysics
|
| Lab |
Wang
|
| Street address |
450 West Drive, CB7295, Lineberger Cancer Center, GREG WANG Lab, RM 31-331
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
|
| Samples (22)
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| Relations |
| BioProject |
PRJNA263448 |
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