|
| Status |
Public on Oct 10, 2014 |
| Title |
MLL-AF9_shEED_3 |
| Sample type |
RNA |
| |
|
| Source name |
MLL-AF9_shEED
|
| Organism |
Mus musculus |
| Characteristics |
strain background: Balb/c
cell line: MLL-AF9-puro
cell type: transformed murine leukemia progenitor cell line
treated with: shEED
|
| Treatment protocol |
Treatment with 3μM of the indicated compounds for 4-5 days, or induction of gene-specific shRNAs (with an inducible TRMPVIR system) for 6 days, followed by total RNA preparation for microarray.
|
| Growth protocol |
Transformed murine leukemia progenitor cell lines were cultured in Opti-MEM base medium supplemented with 10% FBS, 1% antibiotics, 50 μM mercaptoethanol and stem cell factor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the Rneasy Plus Miniprep Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
The cDNA was fragmented and terminally labeled with biotin, using the Affymetrix GeneChip WT Terminal Labeling kit (Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Samples were hybridized with Affymetrix Mouse Gene 2.1 ST Array Strip (Affymetrix) according to the manifacture's instruction.
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
MLL-AF9_shEED_3
|
| Data processing |
Hybridization signals were retrieved and normalized, followed by differential expression analysis and statistical analysis using GeneSpring Analysis Platform GX 12.6 (Agilent Technologies).
probe group file: MoGene-2_1-st.pgf
meta-probeset file: MoGene-2_1-st.mps
|
| |
|
| Submission date |
Oct 09, 2014 |
| Last update date |
Oct 10, 2014 |
| Contact name |
Bowen Xu |
| E-mail(s) |
bowenx@email.unc.edu
|
| Phone |
919-966-5953
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Biochemistry and Biophysics
|
| Lab |
Wang
|
| Street address |
450 West Drive, CB7295, Lineberger Cancer Center, GREG WANG Lab, RM 31-331
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE62198 |
Expression data of MLL-AF9-transformed murine leukemia progenitor cells, following treatment with UNC1999, a dual Ezh1 and Ezh2 inhibitor, or knockdown of EED |
|
