|
| Status |
Public on Nov 27, 2015 |
| Title |
Hippocampus SAHA and Tadalafil. Biological replica 4 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse hippocampi after chronic treatment with SAHA and Tadalafil
|
| Organism |
Mus musculus |
| Characteristics |
strain background: B6.Cg-Tg(APPswe)E1-2Dbo/J
age: 16-18 month-old
sample group: chronic treatment with SAHA and Tadalafil
tissue: hippocampus
|
| Treatment protocol |
Mice were sacrificed by cervical dislocation, brain was extracted and the hippocampus was dissected and kept in dry ice immediately. Tissue was stored at -80 C until RNA extraction.
|
| Growth protocol |
Hippocampus of mouse treated with different solutions during four weeks. The mouse underwent fear conditioning and Morris water maze test during week 3 and 4 of the treatment. Next, mouse reveived a 4-weeks washaout period and underwent to a reversal phase of MWM before sacrificed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was prepared from 300 ng of total RNA using the Ambion® WT Expression Kit. The sense strand cDNA was then fragmented and biotinylated with the Affymetrix GeneChip® WT Terminal Labeling Kit (PN 900671).
|
| |
|
| Hybridization protocol |
Labeled sense cDNA was hybridized to the Affymetrix MoGene-2_0-st chip according to the the manufacturer protocols and using GeneChip® Hybridization, Wash and Stain Kit.
|
| Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000.
|
| Description |
Combi_4
|
| Data processing |
The data were normalized with Bioconductor software using RMA algorithm (Affymetrix GeneChip Mouse Gene 2.0 ST (mogene20stv1cdf from Bioconductor))
|
| |
|
| Submission date |
Oct 10, 2014 |
| Last update date |
Nov 27, 2015 |
| Contact name |
Victor Segura |
| E-mail(s) |
vsegura@unav.es
|
| Organization name |
FIMA
|
| Department |
Unit of Proteomics, Genomics and Bioinformatics
|
| Street address |
Avda. Pío XII, 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE62240 |
A novel approach to Alzheimer’s Disease treatment: HDACs & PDE5 inhibition |
|
