|
Status |
Public on May 16, 2007 |
Title |
Nuc-vs-Genomic-rep2 |
Sample type |
mixed |
|
|
Channel 1 |
Source name |
Nucleosomal rep2
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
See [Yuan et al., Science 309, 626 (2005)]
|
Extracted molecule |
other |
Extraction protocol |
Chromatin digested by MNase. Amplified with a starting amount of up to 75 ng for ChIP samples, using the DNA linear amplification
|
Label |
Cy5
|
Label protocol |
3 μg of aRNA produced from the linear amplification were used to label probe
|
|
|
Channel 2 |
Source name |
Genomic rep2
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
See [Yuan et al., Science 309, 626 (2005)]
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Chromatin sheared by sonication.
|
Label |
Cy3
|
Label protocol |
3 μg of aRNA produced from the linear amplification were used to label probe
|
|
|
|
Hybridization protocol |
was hybridized according to Agilent protocol
|
Scan protocol |
was scanned according to Agilent protocol
|
Description |
Genome-wide levels of nucleosomal DNA. Cells were arrested with alpha factor, chromatin digested by MNase and compared to sonicated input.
|
Data processing |
log2(Cy5/Cy3)
|
|
|
Submission date |
Jan 05, 2007 |
Last update date |
May 16, 2007 |
Contact name |
Tommy Kaplan |
E-mail(s) |
tommy@cs.huji.ac.il
|
Organization name |
Hebrew University
|
Department |
School of Computer Science and Engineering
|
Street address |
Givat Ram Campus
|
City |
Jerusalem |
ZIP/Postal code |
91904 |
Country |
Israel |
|
|
Platform ID |
GPL4131 |
Series (2) |
GSE6668 |
Nucleosome occupancy |
GSE6680 |
Dynamics of replication-independent histone turnover in budding yeast |
|