|
| Status |
Public on Nov 20, 2014 |
| Title |
Eurasier-BI_11731_ARRAY141172 |
| Sample type |
genomic |
| |
|
| Source name |
blood
|
| Organism |
Canis lupus familiaris |
| Characteristics |
tissue: blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from blood using the Puregene DNA purification Kit (Gentra Systems) protocol.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
The array image was acquired using Affymetrix GeneChip® Operating Software.
Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|
| |
|
| Submission date |
Nov 18, 2014 |
| Last update date |
Nov 20, 2014 |
| Contact name |
Hyun Ji Noh |
| E-mail(s) |
noh@broadinstitute.org
|
| Organization name |
Broad Institute
|
| Department |
Genome Sequencing and Analysis
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02141 |
| Country |
USA |
| |
|
| Platform ID |
GPL18091 |
| Series (1) |
| GSE63439 |
Diverse dog breeds genotyped on Affymetrix Canine v2 Arrays |
|
