|
Status |
Public on Nov 20, 2014 |
Title |
Poodle-BI_16925_ARRAY187344 |
Sample type |
genomic |
|
|
Source name |
blood
|
Organism |
Canis lupus familiaris |
Characteristics |
tissue: blood
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted from blood using the Puregene DNA purification Kit (Gentra Systems) protocol.
|
Label |
Biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
Data processing |
The array image was acquired using Affymetrix GeneChip® Operating Software. Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|
|
|
Submission date |
Nov 18, 2014 |
Last update date |
Nov 20, 2014 |
Contact name |
Hyun Ji Noh |
E-mail(s) |
noh@broadinstitute.org
|
Organization name |
Broad Institute
|
Department |
Genome Sequencing and Analysis
|
Street address |
7 Cambridge Center
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02141 |
Country |
USA |
|
|
Platform ID |
GPL18091 |
Series (1) |
GSE63439 |
Diverse dog breeds genotyped on Affymetrix Canine v2 Arrays |
|