|
| Status |
Public on Dec 10, 2015 |
| Title |
CD4+Tcells_CTRL_Patient 5 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+Tcells_Transplanted stable recipient
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4+ T cells isolated from total PBMC
disease state: Control
gender: male
age: 55
treatment: Tacrolimus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CD4+ T cells were isolated from PBMCs using a CD4+ isolation kit (Miltenyi Biotech, Auburn, CA) according to the manufacturer's instructions. Total RNA was extracted with the miRNeasy Mini Kit (Qiagen GmbH, Hilden, Germany). The concentration of total RNA was measured using NANODROP® spectrophotometer ND-1000 (Thermo Scientific, Waltham, MA, US) and RNA integrity was measured using the Agilent 2100 BIOANALYZER® (Agilent Technologies Deutschland GmbH, Waldbronn, Germany) along with the Agilent® RNA 6000 Nano Kit.
|
| Label |
Cy3
|
| Label protocol |
Labeled cRNA was generated using the Low Input Quick Amp Labeling (LIQA) kit (Agilent Technologies, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Labeled cRNA was then hybridized for 17 hours at 65°C on Whole Human Genome Microarray 4x44 K slides, (Agilent Technologies, Santa Clara, CA). After hybridization the slides were washed according to manufacturer’s protocols.
|
| Scan protocol |
The slides were scanned using the High-Resolution Microarray C Scanner (Agilent Technologies).
|
| Data processing |
Data extraction was performed using Agilent Feature Extraction software.
A p-value cut-off of 0.05 and a fold-change cut-off of 2 were applied to identify differentially expressed genes able to discriminate between the study groups.
|
| |
|
| Submission date |
Dec 16, 2014 |
| Last update date |
Dec 10, 2015 |
| Contact name |
Paola Pontrelli |
| E-mail(s) |
paola.pontrelli@uniba.it
|
| Phone |
+390805478868
|
| Organization name |
University of Bari
|
| Department |
Dept. of Precision and Regenerative Medicine and Ionian Area (DIMEPRE-J)
|
| Lab |
Nephrology Unit
|
| Street address |
Piazza G.Cesare 11
|
| City |
Bari |
| ZIP/Postal code |
70124 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE64261 |
Molecular characterization of chronic antibody mediated rejection in kidney transplantation (mRNA CD4) |
|
