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Sample GSM1568249 Query DataSets for GSM1568249
Status Public on Apr 04, 2016
Title Differentiated Keratinocytes (DK), replicate A
Sample type RNA
 
Source name Differentiated Keratinocytes
Organism Homo sapiens
Characteristics cell type: Differentiated Keratinocytes
Treatment protocol none
Growth protocol KP cells were obtained from foreskin biopsies of healthy neonatal donors and expanded by cultivation onto lethally irradiated 3T3-J2 cells in growth FAD medium, a DMEM and Ham’s F12 media mixture (2:1) containing FCS (10%), penicillin-streptomycin (1%), glutamine (2%), insulin(5ug/ml), adenine (24.3ug/ml), hydrocortisone (0.4ug/ml), cholera toxin (50ug/ml), triiodotyronine (2nM). DKs were cultivated in EpiLife serum-free medium (Invitrogen) containing EpiLife defined growth supplements (HKGS, Invitrogen), penicillin-streptomycin (1%) and glutamine (2%).
Extracted molecule total RNA
Extraction protocol total RNA was extracted by RNeasy Mini Kit (QIAGEN) standard protocol and DNAse treated by RNase-Free DNase Set (QIAGEN)
Label biotin
Label protocol cRNA was biotinilated by IVT labeling kit standard protocol (Affymetrix)
 
Hybridization protocol Biotinylated cRNA was hybridized onto GeneChip® HG-U133 Plus 2.0 Arrays according to the protocol supplied by the manufacturer (Affymetrix)
Scan protocol standard Affymetrix protocol
Data processing RMA of .CEL files using affy Bioconductor library. In RMA, PM values have been background adjusted, normalized using quantile normalization, and expression measure calculated using median polish summarization
 
Submission date Dec 17, 2014
Last update date Apr 04, 2016
Contact name Luca Petiti
E-mail(s) luca.petiti@itb.cnr.it
Organization name ITB-CNR
Street address Via Fratelli Cervi, 93
City Segrate
State/province Milan
ZIP/Postal code 20090
Country Italy
 
Platform ID GPL17180
Series (2)
GSE64299 Transcriptional Regulationand Chromatin Dynamics inHuman Epithelial Cell Differentiation (expression)
GSE64328 Transcriptional Regulationand Chromatin Dynamics inHuman Epithelial Cell Differentiation

Data table header descriptions
ID_REF
VALUE RMA-calculated signal intensity (log2)

Data table
ID_REF VALUE
GC00U902522_at 3.097546421
GC00U902726_at 3.190047864
GC00U902727_at 4.083463094
GC00U902728_at 2.655308154
GC00U905129_at 3.223335162
GC00U913730_at 6.481223141
GC00U916295_at 4.879192538
GC00U921637_at 5.336706876
GC00U921664_at 2.831787971
GC00U921668_at 4.611829174
GC00U921857_at 4.393421479
GC00U922209_at 5.048662854
GC00U922231_at 3.170821974
GC00U922298_at 3.078944147
GC00U922307_at 2.79169723
GC00U922384_at 5.069066857
GC00U922430_at 4.71291708
GC00U922457_at 4.718447
GC00U922633_at 4.715133694
GC00U922796_at 4.459222981

Total number of rows: 18862

Table truncated, full table size 495 Kbytes.




Supplementary file Size Download File type/resource
GSM1568249_DK_Pz_1_def.CEL.gz 7.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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