|
| Status |
Public on Jun 30, 2009 |
| Title |
Wt_Fe minus_rep1_1430 |
| Sample type |
RNA |
| |
|
| Source name |
Fetal livers extracted on day E14.5
|
| Organism |
Mus musculus |
| Characteristics |
strain:
Gender:
Age: Embryo E14.5
|
| Treatment protocol |
Wildtype mice were maintianed in iron -deficient diet for 3 months
|
| Growth protocol |
Wildtype mice were maintianed in iron-deficient diet for 3 months.
A state of iron deficiency was induced by placing newborn mice (after weaving) on a low diet containing 5 p.p.m Fe
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted after mice were scarificed and featl livers were taken on day E14.5.
|
| Label |
Biotin
|
| Label protocol |
Total RNA (1 µg) was amplified for the first strand cDNA synthesis using the Affymetrix one-cycle cDNA synthesis protocol. The second strand DNA synthesis, cleanup of double-stranded cDNA, and biotin-labeling of cRNA were carried out according to the Affymetrix manual. The GeneChip Cleanup module was used to purify the double-stranded cDNA and the biotin-labeled cRNA. The cRNA was quantitated using a NanoDrop ND-1000 UV-vis spectrophotometer (NanoDrop Tecnologies, Rockland, DE).
|
| |
|
| Hybridization protocol |
Sample cocktails (200 µl) were used for hybridizations containing 10 µg of cRNA. Microarrays were hybridized overnight, washed in a GeneChip fluidic station 450. scanned using the GeneChip Scanner 3000 as described by Affymetrix.
|
| Scan protocol |
scanned using the GeneChip Scanner 3000 as described by Affymetrix.
|
| Description |
Fetal livers extracted on day E14.5
|
| Data processing |
The .cel files from affymetrix were normalized using GCRMA from S-plus array analyzer module
|
| |
|
| Submission date |
Jan 19, 2007 |
| Last update date |
Dec 29, 2008 |
| Contact name |
Sanchita Bhattacharya |
| E-mail(s) |
SBhattacharya@lbl.gov
|
| Phone |
510-486-6881
|
| Organization name |
MIT
|
| Street address |
1 Cyclotron Rd
|
| City |
Berkely |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (2) |
| GSE2918 |
Feedback control by the human microRNA, let-7, regulates expression of the RNA sielncing enzyme, Dicer |
| GSE6808 |
HRI Protects Erythroid Precursors in iron deficiency and beta-Thalassemia by Maintaining GATA-1 and Fog-1 Expression |
|
