 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 22, 2007 |
| Title |
sulA___U_N0025_r3 |
| Sample type |
RNA |
| |
|
| Source name |
Escherichia coli
|
| Organism |
Escherichia coli |
| Characteristics |
sulA upregulation, amp 50ug/ml, 0.0125% arabinose, 0.025ug/ml norfloxacin OD ~0.3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAEasy with RNAProtect(Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared according to the standard Affymetrix E. coli protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA was hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
|
| Description |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
|
| Data processing |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
|
| |
|
| Submission date |
Jan 22, 2007 |
| Last update date |
Jan 22, 2007 |
| Contact name |
Timothy S Gardner |
| E-mail(s) |
tgardner@bu.edu
|
| URL |
http://gardnerlab.bu.edu/
|
| Organization name |
Boston University
|
| Department |
Biomedical Engineering
|
| Street address |
44 Cummington St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL199 |
| Series (1) |
| GSE6836 |
Large-Scale Mapping and Validation of E. coli Transcriptional Regulation from a Compendium of Expression Profiles. |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
MAS5-calculated Signal intensity |
| ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
| DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| ompX_b0814_at |
3911 |
P |
0.000327 |
| pykF_b1676_at |
2633 |
P |
0.000735 |
| fimD_b4317_at |
27.6 |
A |
0.5 |
| slyB_b1641_at |
3848.6 |
P |
0.000327 |
| IG_1234_1928427_1928480_rev_at |
442.2 |
P |
0.000491 |
| dmsB_b0895_at |
69.3 |
A |
0.181744 |
| IG_69_127588_127911_fwd_f_at |
21350.3 |
P |
0.000147 |
| IG_2143_3432799_3432843_fwd_at |
155.2 |
A |
0.073242 |
| IG_1827_2940590_2940939_rev_at |
85.2 |
A |
0.078906 |
| IG_941_1502890_1504195_fwd_at |
2 |
A |
0.984836 |
| adiA_b4117_at |
97.1 |
A |
0.198363 |
| b1810_at |
194.8 |
P |
0.000893 |
| b1675_at |
184.3 |
P |
0.015164 |
| cysK_b2414_at |
1033.8 |
P |
0.000403 |
| yjeS_b4166_at |
279.9 |
P |
0.020444 |
| IG_2356_3818804_3825086_rev_at |
57.6 |
P |
0.000368 |
| yrfD_b3395_at |
20 |
A |
0.432352 |
| IG_1746_2797635_2798154_fwd_at |
4.4 |
A |
0.927908 |
| IG_1180_1863661_1863749_rev_at |
121.1 |
P |
0.000944 |
| ycbP_b0937_at |
77.5 |
A |
0.537511 |
Total number of rows: 7312
Table truncated, full table size 259 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM157438.CEL.gz |
1.9 Mb |
(ftp)(http) |
CEL |
|
|
|
|
 |
