GEO Publications
Handout
NAR 2024 (latest)
NAR 2002 (original)
All publications
FAQ
MIAME
Email GEO
NCBI
>
GEO
>
Accession Display
Not logged in |
Login
GEO help:
Mouse over screen elements for information.
Scope:
Self
Platform
Samples
Series
Family
Format:
HTML
SOFT
MINiML
Amount:
Brief
Quick
GEO accession:
Sample GSM157579
Query DataSets for GSM157579
Status
Public on Jan 22, 2007
Title
WT_N0025_r1
Sample type
RNA
Source name
Escherichia coli
Organism
Escherichia coli
Characteristics
wild-type cells with 0.025 ug/ml norfloxacin
Extracted molecule
total RNA
Extraction protocol
RNAEasy with RNAProtect(Qiagen)
Label
biotin
Label protocol
Biotinylated cDNA was prepared according to the standard Affymetrix E. coli protocol
Hybridization protocol
Following fragmentation, cDNA was hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Description
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Data processing
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Submission date
Jan 22, 2007
Last update date
Jan 22, 2007
Contact name
Timothy S Gardner
E-mail(s)
tgardner@bu.edu
URL
http://gardnerlab.bu.edu/
Organization name
Boston University
Department
Biomedical Engineering
Street address
44 Cummington St.
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL199
Series (1)
GSE6836
Large-Scale Mapping and Validation of E. coli Transcriptional Regulation from a Compendium of Expression Profiles.
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
ompX_b0814_at
1831.6
P
0.000327
pykF_b1676_at
1442
P
0.000327
fimD_b4317_at
279.5
P
0.002693
slyB_b1641_at
2272.7
P
0.000327
IG_1234_1928427_1928480_rev_at
512.7
P
0.000491
dmsB_b0895_at
383.4
P
0.001571
IG_69_127588_127911_fwd_f_at
8673.6
P
0.000147
IG_2143_3432799_3432843_fwd_at
271.4
P
0.007813
IG_1827_2940590_2940939_rev_at
287.2
P
0.000613
IG_941_1502890_1504195_fwd_at
1.7
A
0.877253
adiA_b4117_at
594.6
P
0.000944
b1810_at
663.2
P
0.000403
b1675_at
554.7
P
0.000944
cysK_b2414_at
1510
P
0.000403
yjeS_b4166_at
744.7
P
0.000603
IG_2356_3818804_3825086_rev_at
321.8
P
0
yrfD_b3395_at
360.5
P
0.001886
IG_1746_2797635_2798154_fwd_at
119.9
P
0.001105
IG_1180_1863661_1863749_rev_at
148.2
P
0.002607
ycbP_b0937_at
335
P
0.042142
Total number of rows:
7312
Table truncated, full table size
258 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM157579.CEL.gz
2.2 Mb
(ftp)
(http)
CEL
|
NLM
|
NIH
|
GEO Help
|
Disclaimer
|
Accessibility
|