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GEO accession:
Sample GSM157614
Query DataSets for GSM157614
Status
Public on Jan 22, 2007
Title
T48_N0025_r3
Sample type
RNA
Source name
Escherichia coli
Organism
Escherichia coli
Characteristics
cells 48 min after treatment by norfloxacin
Extracted molecule
total RNA
Extraction protocol
RNAEasy with RNAProtect(Qiagen)
Label
biotin
Label protocol
Biotinylated cDNA was prepared according to the standard Affymetrix E. coli protocol
Hybridization protocol
Following fragmentation, cDNA was hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Description
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Data processing
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Submission date
Jan 22, 2007
Last update date
Jan 22, 2007
Contact name
Timothy S Gardner
E-mail(s)
tgardner@bu.edu
URL
http://gardnerlab.bu.edu/
Organization name
Boston University
Department
Biomedical Engineering
Street address
44 Cummington St.
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL199
Series (1)
GSE6836
Large-Scale Mapping and Validation of E. coli Transcriptional Regulation from a Compendium of Expression Profiles.
Data table header descriptions
ID_REF
VALUE
MAS5-calculated Signal intensity
ABS_CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE
'detection p-value', p-value that indicates the significance level of the detection call
Data table
ID_REF
VALUE
ABS_CALL
DETECTION P-VALUE
ompX_b0814_at
5168.5
P
0.000327
pykF_b1676_at
324.7
P
0.011548
fimD_b4317_at
40.2
A
0.567648
slyB_b1641_at
1401.5
P
0.000603
IG_1234_1928427_1928480_rev_at
12495.9
P
0.000491
dmsB_b0895_at
73.2
A
0.324781
IG_69_127588_127911_fwd_f_at
29035.3
P
0.000176
IG_2143_3432799_3432843_fwd_at
245.7
A
0.113281
IG_1827_2940590_2940939_rev_at
108.1
P
0.023995
IG_941_1502890_1504195_fwd_at
2.8
A
0.990717
adiA_b4117_at
63.5
A
0.165268
b1810_at
423.4
P
0.001886
b1675_at
88.1
A
0.586954
cysK_b2414_at
600
P
0.000603
yjeS_b4166_at
2811.3
P
0.000403
IG_2356_3818804_3825086_rev_at
140.9
P
3.2e-05
yrfD_b3395_at
26.2
A
0.611787
IG_1746_2797635_2798154_fwd_at
147
P
0.008195
IG_1180_1863661_1863749_rev_at
268
P
0.002143
ycbP_b0937_at
139.5
A
0.318883
Total number of rows:
7312
Table truncated, full table size
258 Kbytes
.
Supplementary file
Size
Download
File type/resource
GSM157614.CEL.gz
1.9 Mb
(ftp)
(http)
CEL
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