|
| Status |
Public on Jan 22, 2007 |
| Title |
ccdB_MG1063_30_r1 |
| Sample type |
RNA |
| |
|
| Source name |
Escherichia coli
|
| Organism |
Escherichia coli |
| Characteristics |
E.coli MG1063 (recA56 = recA-) with ccdB upregulation 30 minutes after induction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAEasy with RNAProtect(Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared according to the standard Affymetrix E. coli protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA was hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
|
| Description |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
|
| Data processing |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
|
| |
|
| Submission date |
Jan 22, 2007 |
| Last update date |
Jan 22, 2007 |
| Contact name |
Timothy S Gardner |
| E-mail(s) |
tgardner@bu.edu
|
| URL |
http://gardnerlab.bu.edu/
|
| Organization name |
Boston University
|
| Department |
Biomedical Engineering
|
| Street address |
44 Cummington St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL199 |
| Series (1) |
| GSE6836 |
Large-Scale Mapping and Validation of E. coli Transcriptional Regulation from a Compendium of Expression Profiles. |
|
