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Sample GSM157661 Query DataSets for GSM157661
Status Public on Jan 22, 2007
Title ik_L2_T4.5_r1
Sample type RNA
 
Source name Escherichia coli
Organism Escherichia coli
Characteristics K12 EMG2 on LB with 0.2 percent glucose, 4.5 hours post-incubation
Extracted molecule total RNA
Extraction protocol RNAEasy with RNAProtect(Qiagen)
Label biotin
Label protocol Biotinylated cDNA was prepared according to the standard Affymetrix E. coli protocol
 
Hybridization protocol Following fragmentation, cDNA was hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Description GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Data processing GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
 
Submission date Jan 22, 2007
Last update date Jan 22, 2007
Contact name Timothy S Gardner
E-mail(s) tgardner@bu.edu
URL http://gardnerlab.bu.edu/
Organization name Boston University
Department Biomedical Engineering
Street address 44 Cummington St.
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL199
Series (1)
GSE6836 Large-Scale Mapping and Validation of E. coli Transcriptional Regulation from a Compendium of Expression Profiles.

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
ompX_b0814_at 8667.1 P 0.000327
pykF_b1676_at 2980.9 P 0.000403
fimD_b4317_at 43.8 A 0.388213
slyB_b1641_at 5705.9 P 0.000327
IG_1234_1928427_1928480_rev_at 140.3 P 0.000491
dmsB_b0895_at 57.6 A 0.069878
IG_69_127588_127911_fwd_f_at 15229.2 P 0.000147
IG_2143_3432799_3432843_fwd_at 335.4 P 0.023438
IG_1827_2940590_2940939_rev_at 62.7 P 0.048099
IG_941_1502890_1504195_fwd_at 2.1 A 0.982268
adiA_b4117_at 68.9 A 0.254899
b1810_at 248.8 P 0.0178
b1675_at 62 A 0.216313
cysK_b2414_at 2108.6 P 0.000403
yjeS_b4166_at 295.6 P 0.030446
IG_2356_3818804_3825086_rev_at 54.8 P 0.000612
yrfD_b3395_at 24.2 A 0.432352
IG_1746_2797635_2798154_fwd_at 3.8 A 0.924354
IG_1180_1863661_1863749_rev_at 97.1 P 0.007827
ycbP_b0937_at 56.2 A 0.462489

Total number of rows: 7312

Table truncated, full table size 258 Kbytes.




Supplementary file Size Download File type/resource
GSM157661.CEL.gz 2.0 Mb (ftp)(http) CEL

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