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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 22, 2007 |
| Title |
ik_L2_T8_r1 |
| Sample type |
RNA |
| |
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| Source name |
Escherichia coli
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| Organism |
Escherichia coli |
| Characteristics |
K12 EMG2 on LB with 0.2 percent glucose, 8 hours post-incubation
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNAEasy with RNAProtect(Qiagen)
|
| Label |
biotin
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| Label protocol |
Biotinylated cDNA was prepared according to the standard Affymetrix E. coli protocol
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| Hybridization protocol |
Following fragmentation, cDNA was hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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| Scan protocol |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
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| Description |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
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| Data processing |
GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
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| |
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| Submission date |
Jan 22, 2007 |
| Last update date |
Jan 22, 2007 |
| Contact name |
Timothy S Gardner |
| E-mail(s) |
tgardner@bu.edu
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| URL |
http://gardnerlab.bu.edu/
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| Organization name |
Boston University
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| Department |
Biomedical Engineering
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| Street address |
44 Cummington St.
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platform ID |
GPL199 |
| Series (1) |
| GSE6836 |
Large-Scale Mapping and Validation of E. coli Transcriptional Regulation from a Compendium of Expression Profiles. |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
MAS5-calculated Signal intensity |
| ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
| DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| ompX_b0814_at |
1903.9 |
P |
0.000327 |
| pykF_b1676_at |
1596.9 |
P |
0.000893 |
| fimD_b4317_at |
53.4 |
A |
0.654528 |
| slyB_b1641_at |
16376 |
P |
0.000327 |
| IG_1234_1928427_1928480_rev_at |
67.5 |
P |
0.000762 |
| dmsB_b0895_at |
272.7 |
P |
0.04977 |
| IG_69_127588_127911_fwd_f_at |
4257 |
P |
0.000147 |
| IG_2143_3432799_3432843_fwd_at |
165.6 |
A |
0.113281 |
| IG_1827_2940590_2940939_rev_at |
78.4 |
A |
0.099061 |
| IG_941_1502890_1504195_fwd_at |
4.6 |
A |
0.96798 |
| adiA_b4117_at |
114.4 |
A |
0.135974 |
| b1810_at |
362.3 |
P |
0.009939 |
| b1675_at |
53 |
A |
0.635055 |
| cysK_b2414_at |
6116.1 |
P |
0.000327 |
| yjeS_b4166_at |
358.5 |
P |
0.044201 |
| IG_2356_3818804_3825086_rev_at |
69.8 |
P |
0.035327 |
| yrfD_b3395_at |
46.2 |
A |
0.522646 |
| IG_1746_2797635_2798154_fwd_at |
5.3 |
A |
0.963324 |
| IG_1180_1863661_1863749_rev_at |
157.9 |
P |
0.015164 |
| ycbP_b0937_at |
319.4 |
A |
0.165268 |
Total number of rows: 7312
Table truncated, full table size 259 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM157665.CEL.gz |
1.9 Mb |
(ftp)(http) |
CEL |
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