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Sample GSM157672 Query DataSets for GSM157672
Status Public on Jan 22, 2007
Title ik_H2_T5.5_r1
Sample type RNA
 
Source name Escherichia coli
Organism Escherichia coli
Characteristics K12 EMG2 on LB with 0.4 percent glucose, 5.5 hours post-incubation
Extracted molecule total RNA
Extraction protocol RNAEasy with RNAProtect(Qiagen)
Label biotin
Label protocol Biotinylated cDNA was prepared according to the standard Affymetrix E. coli protocol
 
Hybridization protocol Following fragmentation, cDNA was hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Description GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
Data processing GeneChips were washed and stained with an FS450 fluidics machine and scanned using a GeneChip Scanner 3000
 
Submission date Jan 22, 2007
Last update date Jan 22, 2007
Contact name Timothy S Gardner
E-mail(s) tgardner@bu.edu
URL http://gardnerlab.bu.edu/
Organization name Boston University
Department Biomedical Engineering
Street address 44 Cummington St.
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL199
Series (1)
GSE6836 Large-Scale Mapping and Validation of E. coli Transcriptional Regulation from a Compendium of Expression Profiles.

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
ompX_b0814_at 4841.3 P 0.000327
pykF_b1676_at 1297.9 P 0.000403
fimD_b4317_at 34.8 A 0.266065
slyB_b1641_at 10080.5 P 0.000327
IG_1234_1928427_1928480_rev_at 196.1 P 0.000491
dmsB_b0895_at 110.2 P 0.005297
IG_69_127588_127911_fwd_f_at 12665.7 P 0.000147
IG_2143_3432799_3432843_fwd_at 248.9 P 0.007813
IG_1827_2940590_2940939_rev_at 72.7 P 0.015164
IG_941_1502890_1504195_fwd_at 2.3 A 0.900939
adiA_b4117_at 898.5 P 0.000944
b1810_at 364.2 P 0.000893
b1675_at 33.2 A 0.364945
cysK_b2414_at 22504.5 P 0.000327
yjeS_b4166_at 328.5 P 0.003799
IG_2356_3818804_3825086_rev_at 56 P 4.7e-05
yrfD_b3395_at 219.8 A 0.127994
IG_1746_2797635_2798154_fwd_at 106.7 P 0.002314
IG_1180_1863661_1863749_rev_at 48.6 P 0.010972
ycbP_b0937_at 126 A 0.181343

Total number of rows: 7312

Table truncated, full table size 258 Kbytes.




Supplementary file Size Download File type/resource
GSM157672.CEL.gz 2.0 Mb (ftp)(http) CEL

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