|
| Status |
Public on Jan 01, 2018 |
| Title |
JQ1+ (UJ006) |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
cell type: B cells
subset: CD43-
strain: C57BL/6
culture: LPS+ IL4 plus DMSO stimulated B cells for 3 days
treatment: JQ1+
|
| Treatment protocol |
Treated with Control, JQ1+(80nM) and Flavopiridol (30nM) for 3 days
|
| Growth protocol |
all cells were cultured in RPMI1640 medium plus LPS+ IL4 for 3 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol, rRNA was depleted with the Ribo-Zero rRNA removal kit (Epicentre)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
55 TO 60 MILLION 2x100 BP PAIRED END RAW PASSING FILTERS READS SEQUENCED ON ILLUMINA HISEQ 2000 V3 INSTRUMENT
map reads onto reference genome mm9
estimate normalized expression level (RPKM/FPKM) of known genes and transcripts
Genome_build: mm9
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Jan 13, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Guoxin Liang |
| E-mail(s) |
gxliang@cmu.edu.cn
|
| Organization name |
China Medical University
|
| Department |
AIDS research center
|
| Street address |
No.155 Nanjing North street
|
| City |
Shenyang |
| State/province |
Liaoning |
| ZIP/Postal code |
110001 |
| Country |
China |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE64936 |
RNA profiling for mouse B cells by drug treatment |
|
| Relations |
| BioSample |
SAMN03283230 |
| SRA |
SRX840267 |
