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| Status |
Public on Jan 28, 2016 |
| Title |
Testis |
| Sample type |
SRA |
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| Source name |
Testis
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| Organism |
Takifugu rubripes |
| Characteristics |
tissue: Testis
genotype: wild type
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a mirVana miRNA Isolation Kit (Applied Biosystems).
Nine small RNA libraries were constructed, using Small 415 RNA Library Preparation for SOLiD Sequencing (Applied Biosystems)
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| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
AB SOLiD System 3.0 |
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| Data processing |
low quality reads and 3′ sequencing adapters were eliminated and trimmed, respectively, by our own Perl script.
Small RNAs (18–25 nt) were Blast-searched against mature miRNAs deposited in miRBase 19.0 (www.mirbase.org/), using CLC genomics workbench software (CLC bio, Aarhus, Denmark).
Genome_build: miRBase 19.0
Supplementary_files_format_and_content: tab-delimited text files containing sequence, expression values, length, count, name, resource, match type and mismatches.
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| Submission date |
Jan 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
chaninya wongwarangkana |
| E-mail(s) |
tarnjew@gmail.com
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| Organization name |
the university of tokyo
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| Department |
aquatic bioscience
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| Lab |
aquatic molecular biology and biotechnology
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| Street address |
1-1-1 Yayoi
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| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
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| Platform ID |
GPL19713 |
| Series (1) |
| GSE65404 |
Deep sequencing, profiling and detailed annotation of miRNAs in Takifugu rubripes |
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| Relations |
| BioSample |
SAMN03299892 |
| SRA |
SRX857212 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1595666_FTM_18_25nt_annotated_pool.txt.gz |
12.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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