|
Status |
Public on Mar 19, 2015 |
Title |
125AA-EL36_water_stress-2010_46 |
Sample type |
RNA |
|
|
Source name |
berry
|
Organism |
Vitis vinifera |
Characteristics |
tissue: berry scion cultivar: pinot noir rootstock: 125AA developmental stage: EL36 treatment: water stress year: 2010
|
Treatment protocol |
Two water conditions were established for the vines grafted on the two rootstocks: control (C) and water stress treatment (S). To impose water stress, half the rows of the vineyard chosen were covered with a removable plastic film from flowering onwards, covering completely the width of the inter-row on both sides of the plants during rainy days. Water stress was applied to the upper part of the vineyard (slope with 6-7% of inclination) and the control was situated at the lower part.
|
Growth protocol |
Grape berries from Pinot noir (Vitis vinifera) grafted on rootstocks Richter 110 (V. rupestris x V. berlandieri) and Kober 125AA (V. riparia x V. berlandieri), were sampled from field-grown vines at two different developmental stages (E-L 35 and E-L 36) according to the modified Eichhorn and Lorenz developmental scale24, during two growing seasons (2009 and 2010). Vines were trained using a vertical shoot positioning system. The experimental vineyard belongs to the University of Geisenheim (49°59’N, 7°57’W, Germany). Twenty rows of Pinot noir were selected, with an average of 17 plants per row.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from berries of Pinot noir grafted onto 110R and 125AA was extracted as described by Reid et al., 2006 (Reid KE, Olsson N, Schlosser J, Peng F, Lund ST. An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development. BMC Plant Biology 2006; 6: 27.). Seeds of each berry were removed before grinding in liquid nitrogen. Total RNA was subjected to DNA digestion with 5 units of RNase-free DNase I (Promega) for 1 h at 37°C. RNA integrity was measured at 260 nm with a micro-spectrophotometer (NanoDrop 200C, NanoDrop products, Wilmington, USA) and visualized by electrophoresis on 1.2% agarose gels. RNA quality was also confirmed using a lab-on-chip (2100 Bioanalyzer, Agilent Technologies, Santa Clara, USA).
|
Label |
Cy3
|
Label protocol |
Labelling, hybridization and scanning were done by Genotoul Biopuces plateform (Toulouse, France).
|
|
|
Hybridization protocol |
Labelling, hybridization and scanning were done by Genotoul Biopuces plateform (Toulouse, France).
|
Scan protocol |
Labelling, hybridization and scanning were done by Genotoul Biopuces plateform (Toulouse, France).
|
Data processing |
Data were RMA normalized using R/Bioconductor.
|
|
|
Submission date |
Feb 27, 2015 |
Last update date |
Mar 20, 2015 |
Contact name |
Christian Kappel |
E-mail(s) |
christian.kappel@uni-potsdam.de
|
Phone |
+49-331-9775580
|
Organization name |
Universität Potsdam
|
Department |
Institut für Biochemie und Biologie
|
Street address |
Karl-Liebknecht-Str. 24-25, Haus 26
|
City |
Potsdam |
ZIP/Postal code |
14476 |
Country |
Germany |
|
|
Platform ID |
GPL17894 |
Series (1) |
GSE66391 |
Water limitation and rootstock genotype interact to alter grape berry metabolism through transcriptome reprogramming |
|