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Status |
Public on Jun 01, 2020 |
Title |
neofascia from native environment of patient CS_10 |
Sample type |
RNA |
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Source name |
native tissue sample
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Organism |
Homo sapiens |
Characteristics |
patient: CS_10 environment: native healer: good rna integrity number [rin]: 2.4
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Treatment protocol |
Bioreactor culture was performed in the presence of mesh A (Parietex) or mesh B (ULTRAPRO).
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Growth protocol |
Fibroblasts were generated by collagenase digestion for 16h in DMEM+10% fetal bovine serum (FBS)+1% Penicillin/Streptomycin (Pen/Strep) at 37°C, after sieving, life cells were precultured in DMEM+10% FBS+1%Pen/Strep; for bioreactor culture, 750,000 cells were embedded in 5ml collagen type 1 gel matrix, fed with 5ml DMEM+10% FBS+1%Pen/Strep and incubated for 14 days at 37°C.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the miRNeasy kit (Qiagen) according to the manufactuer's instractions.
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Label |
biotin
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Label protocol |
100ng of total RNA were amplified and labeled with the IVT Express Kit (Affymetrix); all steps were performed according to the manufacturer's protocols.
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Hybridization protocol |
130µl hybridization mix prepared from labeled cRNA fragments was hybridized for 16h at 45°C and 60 rpm to GeneChip PrimeView arrays. Microarrays were washed and stained in the Affymetrix Fluidics Station 450 using script FS450_0002.
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Scan protocol |
Microarrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix).
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Description |
neofascia from native environment of patient CS_10
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Data processing |
Data were preprocessed in the R v3.0.1 environment with Bioconductor package affy; background correction, normalization and probeset summary were performed with the rma command using default parameter settings.
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Submission date |
Mar 12, 2015 |
Last update date |
Jun 01, 2020 |
Contact name |
Claus Juergen Scholz |
Organization name |
University Hospital Würzburg
|
Department |
Core Unit SysMed
|
Street address |
Josef-Schneider-Str. 2
|
City |
Würzburg |
ZIP/Postal code |
97080 |
Country |
Germany |
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|
Platform ID |
GPL15207 |
Series (1) |
GSE66818 |
Global gene expression profiling of tissue engineered neofascia of hernia patients with good and bad healing characterisitics. |
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