|
| Status |
Public on Jun 01, 2020 |
| Title |
neofascia from native environment of patient CS_10 |
| Sample type |
RNA |
| |
|
| Source name |
native tissue sample
|
| Organism |
Homo sapiens |
| Characteristics |
patient: CS_10
environment: native
healer: good
rna integrity number [rin]: 2.4
|
| Treatment protocol |
Bioreactor culture was performed in the presence of mesh A (Parietex) or mesh B (ULTRAPRO).
|
| Growth protocol |
Fibroblasts were generated by collagenase digestion for 16h in DMEM+10% fetal bovine serum (FBS)+1% Penicillin/Streptomycin (Pen/Strep) at 37°C, after sieving, life cells were precultured in DMEM+10% FBS+1%Pen/Strep; for bioreactor culture, 750,000 cells were embedded in 5ml collagen type 1 gel matrix, fed with 5ml DMEM+10% FBS+1%Pen/Strep and incubated for 14 days at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the miRNeasy kit (Qiagen) according to the manufactuer's instractions.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA were amplified and labeled with the IVT Express Kit (Affymetrix); all steps were performed according to the manufacturer's protocols.
|
| |
|
| Hybridization protocol |
130µl hybridization mix prepared from labeled cRNA fragments was hybridized for 16h at 45°C and 60 rpm to GeneChip PrimeView arrays. Microarrays were washed and stained in the Affymetrix Fluidics Station 450 using script FS450_0002.
|
| Scan protocol |
Microarrays were scanned using a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
neofascia from native environment of patient CS_10
|
| Data processing |
Data were preprocessed in the R v3.0.1 environment with Bioconductor package affy; background correction, normalization and probeset summary were performed with the rma command using default parameter settings.
|
| |
|
| Submission date |
Mar 12, 2015 |
| Last update date |
Jun 01, 2020 |
| Contact name |
Claus Juergen Scholz |
| Organization name |
University Hospital Würzburg
|
| Department |
Core Unit SysMed
|
| Street address |
Josef-Schneider-Str. 2
|
| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE66818 |
Global gene expression profiling of tissue engineered neofascia of hernia patients with good and bad healing characterisitics. |
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