|
| Status |
Public on Aug 09, 2016 |
| Title |
CBS138 delta-vph2 pH5.0 rep.2 |
| Sample type |
RNA |
| |
|
| Source name |
Candida glabrata CBS138 delta-vph2
|
| Organism |
Nakaseomyces glabratus CBS 138 |
| Characteristics |
genotype/variation: delta-vph2
phenotype: loss of V-ATPase activity
growth protocol: pH 5.0
|
| Treatment protocol |
Candida glabrata CBS138 delta-vph2 was grown to log phase at 30°C in SC broth (pH5.0 and 7.4).
|
| Growth protocol |
Candida glabrata CBS138 was grown to log phase at 30°C in SC broth (pH5.0 and 7.4).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using RNeasy Mini Kit (Qiagen, Valencia, CA). The quality of RNA was checked with a RNA 6000 Nano Kit and Agilent 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
This sample is of Candida glabrata CBS138 delta-vph2 in SC broth (pH5.0). It is the second of two delta-vph2 mutant biological replicates used in this experiment, each from separate cultures.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Mar 17, 2015 |
| Last update date |
Aug 09, 2016 |
| Contact name |
Hiroshi Nishikawa |
| E-mail(s) |
hiroshi_nishikawa@toyama-chemical.co.jp
|
| Organization name |
Nagasaki University Graduate School of Biomedical Sciences
|
| Street address |
1-7-1 Sakamoto
|
| City |
Nagasaki |
| ZIP/Postal code |
852-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19901 |
| Series (1) |
| GSE66984 |
Expression analysis of Candida glabrata CBS138 delta-vph2 mutant |
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