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Sample GSM1650447 Query DataSets for GSM1650447
Status Public on Jun 17, 2015
Title l T2_6
Sample type RNA
 
Source name Liver
Organism Danio rerio
Characteristics tissue: Liver
treatment: low chlorothalonil
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Trizol protocol for total RNA
Label Cy3
Label protocol Agilent one color protocol for Cy3
 
Hybridization protocol Agilent one color protocol; Microarrays were washed the following day according to the supplemental Agilent protocol “Preventing Ozone-Related Problems”. Slides were washed in GE Wash Buffer 1 at room temperature for 1 min, followed by GE Wash Buffer 2 at 37ºC for 1 min, acetonitrile wash at room temperature for 1 min, and Stabilizing and Drying Solution wash at room temperature for 30 s.
Scan protocol Microarrays were kept in the dark until scanned at 5 μm at both 10 and 100 PMT (Agilent G2505 B Microarray Scanner). Agilent Feature Extraction Software 10.7.3.1 was used to extract raw data.
Data processing Normalized signal intensity provided was done by quanitle normalization; data were filterd at 1.5 prior to ANOVA
 
Submission date Apr 06, 2015
Last update date Nov 13, 2022
Contact name Christopher Martyniuk
E-mail(s) cmartyn@ufl.edu
Phone 3523173905
Organization name University of Florida
Street address 8891 SW 79th Avenue
City Gainesville
State/province FL
ZIP/Postal code 32608
Country USA
 
Platform ID GPL14688
Series (1)
GSE67600 Differential hepatic gene expression in zebrafish (Danio rerio) exposed to the fungicide chlorothalonil reveals estrogenicity

Data table header descriptions
ID_REF
VALUE quantile normalized signal

Data table
ID_REF VALUE
1 13.9684831
2 0.59971819
3 0.60414306
4 0.60823762
5 0.61165204
6 0.61337843
7 0.61652628
8 0.61878164
9 0.62166497
10 0.62371729
11 0.62646402
12 0.62830505
13 0.63018808
14 0.63192328
15 2.30777734
16 5.9816094
17 2.33523567
18 1.14186946
19 8.78878682
20 10.0304913

Total number of rows: 45220

Table truncated, full table size 735 Kbytes.




Supplementary file Size Download File type/resource
GSM1650447_US10173810_252643711013_S01_GE1_107_Sep09_1_3.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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