|
Status |
Public on Jun 17, 2015 |
Title |
l T2_6 |
Sample type |
RNA |
|
|
Source name |
Liver
|
Organism |
Danio rerio |
Characteristics |
tissue: Liver treatment: low chlorothalonil
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol protocol for total RNA
|
Label |
Cy3
|
Label protocol |
Agilent one color protocol for Cy3
|
|
|
Hybridization protocol |
Agilent one color protocol; Microarrays were washed the following day according to the supplemental Agilent protocol “Preventing Ozone-Related Problems”. Slides were washed in GE Wash Buffer 1 at room temperature for 1 min, followed by GE Wash Buffer 2 at 37ºC for 1 min, acetonitrile wash at room temperature for 1 min, and Stabilizing and Drying Solution wash at room temperature for 30 s.
|
Scan protocol |
Microarrays were kept in the dark until scanned at 5 μm at both 10 and 100 PMT (Agilent G2505 B Microarray Scanner). Agilent Feature Extraction Software 10.7.3.1 was used to extract raw data.
|
Data processing |
Normalized signal intensity provided was done by quanitle normalization; data were filterd at 1.5 prior to ANOVA
|
|
|
Submission date |
Apr 06, 2015 |
Last update date |
Nov 13, 2022 |
Contact name |
Christopher Martyniuk |
E-mail(s) |
cmartyn@ufl.edu
|
Phone |
3523173905
|
Organization name |
University of Florida
|
Street address |
8891 SW 79th Avenue
|
City |
Gainesville |
State/province |
FL |
ZIP/Postal code |
32608 |
Country |
USA |
|
|
Platform ID |
GPL14688 |
Series (1) |
GSE67600 |
Differential hepatic gene expression in zebrafish (Danio rerio) exposed to the fungicide chlorothalonil reveals estrogenicity |
|