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Sample GSM1673692 Query DataSets for GSM1673692
Status Public on Mar 11, 2016
Title YRA1∆i, biological rep. 1
Sample type RNA
 
Source name Total RNA from W303 cells overexpressing YRA1
Organism Saccharomyces cerevisiae
Characteristics strain: W303-1A + pRS413::GAL-YRA1∆i
genotype: MATa ade2-1 can1-100 trp1-1 his3-11,15 leu2-3,112 ura3-1 + pRS413::GAL-YRA1Δi
Growth protocol Each strain was growing in minimal medium supplemented with raffinose and shifted for four hours to galactose (2%)
Extracted molecule total RNA
Extraction protocol Total RNA was isolated with the Rneasy Midi kit (QIAGEN)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression Analysis Technical Manual, 2004, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Yeast Genome 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression Analysis Technical Manual, 2004, Affymetrix.
Scan protocol Microarrays were scanned using GeneChip® Scanner 3000 7G
Description Gene expression data from mid-log yeast cells in minimal medium galactose (2%)
Data processing The data were normalized by RMA (Robust Microarray Average) to allow direct comparison between expression values in different experiments. Statistical analysis was performed by LIMMA (Linear Models for Microarray Analysis) using the program affylmGUI. Genes showing at least 1.5-fold expression change compared to wild-type strain with a p-value FDR < 0.01, were considered as altered. Expression values were obtained with the anti-log of RMA values
 
Submission date May 04, 2015
Last update date Mar 12, 2016
Contact name Andrés Aguilera
Organization name CABIMER, Universidad de Sevilla
Department Genome BIology
Lab Genome Instability & Cancer
Street address Av. Américo Vespucio 24
City Seville
ZIP/Postal code 41092
Country Spain
 
Platform ID GPL2529
Series (2)
GSE68487 Expression data from Saccharomyces cerevisae strains overexpressing RNA-binding hnRNP Yra1
GSE68488 YRA1 overexpression microarray and ChIP-chip

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
1769308_at 10.83972097
1769309_at 3.790720754
1769310_at 5.194153347
1769311_at 12.34048786
1769312_at 10.13247117
1769313_at 11.22310044
1769314_at 12.6868002
1769315_at 3.002368112
1769316_s_at 3.627049293
1769317_at 10.86388996
1769318_at 3.612152398
1769319_at 12.24138761
1769320_at 9.654522391
1769321_at 3.612169857
1769322_s_at 9.273762838
1769323_at 10.94217949
1769324_at 10.51241521
1769325_at 8.944416425
1769326_at 4.089322287
1769327_at 3.123459346

Total number of rows: 10928

Table truncated, full table size 246 Kbytes.




Supplementary file Size Download File type/resource
GSM1673692_SGavalda4_DELTAi1.CEL.gz 1.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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