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Status |
Public on Mar 11, 2016 |
Title |
YRA1∆i, biological rep. 1 |
Sample type |
RNA |
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Source name |
Total RNA from W303 cells overexpressing YRA1
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Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: W303-1A + pRS413::GAL-YRA1∆i genotype: MATa ade2-1 can1-100 trp1-1 his3-11,15 leu2-3,112 ura3-1 + pRS413::GAL-YRA1Δi
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Growth protocol |
Each strain was growing in minimal medium supplemented with raffinose and shifted for four hours to galactose (2%)
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with the Rneasy Midi kit (QIAGEN)
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA One-Cycle Target Labeling Assays (GeneChip® Expression Analysis Technical Manual, 2004, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip® Yeast Genome 2.0 Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 according to GeneChip® Expression Analysis Technical Manual, 2004, Affymetrix.
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Scan protocol |
Microarrays were scanned using GeneChip® Scanner 3000 7G
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Description |
Gene expression data from mid-log yeast cells in minimal medium galactose (2%)
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Data processing |
The data were normalized by RMA (Robust Microarray Average) to allow direct comparison between expression values in different experiments. Statistical analysis was performed by LIMMA (Linear Models for Microarray Analysis) using the program affylmGUI. Genes showing at least 1.5-fold expression change compared to wild-type strain with a p-value FDR < 0.01, were considered as altered. Expression values were obtained with the anti-log of RMA values
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Submission date |
May 04, 2015 |
Last update date |
Mar 12, 2016 |
Contact name |
Andrés Aguilera |
Organization name |
CABIMER, Universidad de Sevilla
|
Department |
Genome BIology
|
Lab |
Genome Instability & Cancer
|
Street address |
Av. Américo Vespucio 24
|
City |
Seville |
ZIP/Postal code |
41092 |
Country |
Spain |
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Platform ID |
GPL2529 |
Series (2) |
GSE68487 |
Expression data from Saccharomyces cerevisae strains overexpressing RNA-binding hnRNP Yra1 |
GSE68488 |
YRA1 overexpression microarray and ChIP-chip |
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