GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Sample GSM1705452

Query DataSets for GSM1705452

Status

Public on Jun 10, 2015

Title

Serum_Healthy-C26_rep1

Sample type

other

Source name

Serum

Organism

Homo sapiens

Characteristics

diagnosis: Healthy
proliferative nephritis: N/A

Treatment protocol

N/A

Growth protocol

N/A

Extracted molecule

other

Extraction protocol

N/A

Label

Goat anti-Human IgG conjugated to Cy5 (Jackson Immunoresearch, West Grove, PA)

Label protocol

N/A

Hybridization protocol

Serum was diluted 1:200 from stocks (maintained at -80 deg. C. in) in PBS + 0.5% Tween 20 (Sigma-Aldrich, St. Louis, MO) and 3% fetal calf serum (Omega Scientific, Tarzana CA). Post primary incubation, washed arrays were incubated with Goat anti-Human IgG conjugated to Cy5 (Jackson Immunoresearch, West Grove, PA).

Scan protocol

Arrays were scanned using an Axon digital scanning system (Molecular Devices, Sunnyvale, CA)

Data processing

Array images were analyzed using GenePix 6.0 software (Molecular Devices)

Submission date

Jun 08, 2015

Last update date

Jun 11, 2015

Contact name

Jamie Haddon

Organization name

Stanford University

Department

Immunology & Rheumatology

Street address

CCSR Rm 2250, 269 Campus Drive West

City

Stanford

State/province

ZIP/Postal code

94305

Country

USA

Platform ID

GPL20296

Series (1)

GSE69662

Autoantigen microarrays reveal autoantibodies associated with proliferative nephritis and active disease in pediatric systemic lupus erythematosus

Data table header descriptions
ID_REF
VALUE	Median Fluorescence intensity (MFI) of 635 (Cy5) channel minus local background. Negative MFI minus background signals were set to zero.

Data table
ID_REF	VALUE
1	0
2	6.5
3	0
4	0
5	243
6	0
7	0
8	0
9	273.5
10	156.5
11	36
12	234.5
13	4.5
14	0
15	332
16	208
17	0
18	1395
19	23
20	218.5

Total number of rows: 155

Table truncated, full table size 1 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM1705452_108-C26_0635.gpr.gz	46.5 Kb	(ftp)(http)	GPR
Processed data included within Sample table