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Status |
Public on Mar 15, 2007 |
Title |
Beaupré leaves mock-inoculated with water (control, T48 Bpre_T48_Rep1) |
Sample type |
RNA |
|
|
Source name |
Beaupré leaves mock-inoculated with water, 48 hours post-inoculation (control, T48)
|
Organisms |
Populus trichocarpa; Populus deltoides |
Characteristics |
Detached leaves of Populus trichocarpa x Populus deltoides 'Beaupré' cuttings grown in greenhouses were mock-inoculated with water, maintained in vitro under constant illumination at 24°C and harvested 48 hours post-inoculation
|
Biomaterial provider |
Institut National de la Recherche Agronomique (INRA Nancy, France)
|
Extracted molecule |
total RNA |
Extraction protocol |
Leaves were ground in liquid nitrogen, bead beat in QIAgen buffer RLT and then RNA were isolated with QIAgen RNeasy kit
|
Label |
Cy3
|
Label protocol |
Labelling by NimbleGen Company
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|
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Hybridization protocol |
Hybridization by NimbleGen Company
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Scan protocol |
Scanning by NimbleGen Company
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Description |
Procedure for spot detection was performed by NimbleGen Company
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Data processing |
Expression values reflect accross-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA) expressed as log2. For information on RMA see Biostatistics 2003 Apr; 4(2): 249-264
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Submission date |
Feb 21, 2007 |
Last update date |
Feb 21, 2007 |
Contact name |
Sebastien Duplessis |
Organization name |
INRA
|
Department |
UMR 1136 IAM
|
Lab |
Ecogenomics of interactions
|
Street address |
INRA Centre Grand Est - Nancy
|
City |
Champenoux |
ZIP/Postal code |
54280 |
Country |
France |
|
|
Platform ID |
GPL2699 |
Series (1) |
GSE7098 |
Identification of rust-induced genes in poplar using Populus 2.0 NimbleGen oligoarray |
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