|
| Status |
Public on Mar 15, 2007 |
| Title |
Beaupré leaves inoculated with Melampsora larici-populina 98AG31 virulent strain (Compatible, C48 Bpre_C48_Rep3) |
| Sample type |
RNA |
| |
|
| Source name |
Beaupré leaves inoculated with Melampsora larici-populina 98AG31 virulent strain, 48 hours post-inoculation (Compatible, C48)
|
| Organisms |
Populus trichocarpa; Populus deltoides |
| Characteristics |
Detached leaves of Populus trichocarpa x Populus deltoides 'Beaupré' cuttings grown in greenhouses were inoculated with Melampsora larici-populina 98AG31 virulent strain (100000 spores/ml), maintained in vitro under constant illumination at 24°C and harvested 48 hours post-inoculation
|
| Biomaterial provider |
Institut National de la Recherche Agronomique (INRA Nancy, France)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Leaves were ground in liquid nitrogen, bead beat in QIAgen buffer RLT and then RNA were isolated with QIAgen RNeasy kit
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company
|
| Scan protocol |
Scanning by NimbleGen Company
|
| Description |
Procedure for spot detection was performed by NimbleGen Company
|
| Data processing |
Expression values reflect accross-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA) expressed as log2. For information on RMA see Biostatistics 2003 Apr; 4(2): 249-264
|
| |
|
| Submission date |
Feb 21, 2007 |
| Last update date |
Feb 21, 2007 |
| Contact name |
Sebastien Duplessis |
| Organization name |
INRA
|
| Department |
UMR 1136 IAM
|
| Lab |
Ecogenomics of interactions
|
| Street address |
INRA Centre Grand Est - Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL2699 |
| Series (1) |
| GSE7098 |
Identification of rust-induced genes in poplar using Populus 2.0 NimbleGen oligoarray |
|
