|
| Status |
Public on Aug 03, 2015 |
| Title |
non-Tg |
| Sample type |
RNA |
| |
|
| Source name |
cTECs from 5 non-Tg
|
| Organism |
Mus musculus |
| Characteristics |
sample type: NOD mice at day 7
cell type: cortical thymic epithelial cells
genotype: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using an RNeasy Mini kit (QIAGEN) according to the manufacturer's instructions. The quantity and qualty of the RNA was monitored using the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis and amplification was performed using Ovation One-Direct System (NuGEN) using 400 pg of purified total RNA . 5.5 ug of cDNA was fragmented and end-labeled with Encore Biotin Module (TaKaRa). Fragmented cDNA was monitored with the Agilent 2100 Bioanalyzer.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat the hybridization cocktails at 99 °C for 2 minutes, then 45 °C for 5 minutes • Transfer 80 μl of cocktail to Mouse Gene 1.0ST Array (Affymetrix). • Hybridize 20 hours at 45° at 60 rpm.
|
| Scan protocol |
GeneChips were washed and stained immediately after completion of hybridization. Fluidics Script Protocol is FS450_0007. GeneChips were scanned using the Scanner 3000 7G (Affymetrix), and CEL files were generated.
|
| Data processing |
The scanned images were analyzed with GeneSpring GX 13.0 ( Agilent Technologies).
|
| |
|
| Submission date |
Jun 26, 2015 |
| Last update date |
Aug 03, 2015 |
| Contact name |
Mitsuru Matsumoto |
| E-mail(s) |
mitsuru@tokushima-u.ac.jp
|
| Organization name |
Institute for Enzyme Research, Tokushima University
|
| Department |
Division of Molecular Immunology
|
| Street address |
3-18-15 Kuramoto
|
| City |
Tokushima |
| ZIP/Postal code |
770-8503 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE70326 |
Expression data from cortical thymic epithelial cells ectopically expressing Aire |
|
