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Sample GSM180975 Query DataSets for GSM180975
Status Public on Jun 30, 2007
Title HSPH_MIPS_murlungs_430_Particles1
Sample type RNA
 
Source name Total lung RNA of pregnant mouse exposed to PBS insufflation
Organism Mus musculus
Characteristics Strain: BALB/c
Gender: female
Gestation: 14 days
Exposure: PBS insufflation control
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy with on-column DNAse digestion and dual elution.
Label biotin
Label protocol For each experimental sample, RNA quality was assessed by RNA Nano LabChip analysis on an Agilent Bioanalyzer 2100. Concentrations may also be determined using a NanoDrop 1000 spectrophotometer. Under standard conditions processing of RNAs for GeneChip Analysis was in accordance with methods described in the Affymetrix GeneChip Expression Analysis Technical Manual, revision four, as subsequently detailed. Synthesis of cDNA first and second strand is performed using the GeneChip Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (P/N 900431). Cleanup of the double stranded product is carried according to standard Affymetrix protocols using the Affymetrix GeneChip Cleanup Module (Affymetrix Catalog # 900371).
In vitro transcription (IVT) is performed using the GeneChip Expression Amplification Reagents kit- 30 reactions (P/N 900449) and is carried out according to the standard Affymetrix protocols and quantification of the IVT samples is carried out on a Bio-Tek UV Plate Reader.
 
Hybridization protocol Hybridization is carried out according the Affymetrix GeneChip® Manual. Twenty micrograms of IVT material is the nominal amount used on the GeneChip® arrays. Affymetrix hybridization ovens are used to incubate the arrays at a constant temperature of 45oC overnight.
Scan protocol Preparation of microarrays for scanning is carried out with Affymetrix appropriate wash protocols matched to the specific chip type on a Model 450 Fluidics station. Affymetrix GeneChip Operating Software (GCOS) operating system controls the Fluidics station process.
Scanning is carried out on a GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System. Chip library files specific to each array and necessary for scan interpretation are stored on the computer workstation controlling the scanner and are updated regularly as necessary when updates are made available from Affymetrix. Collected research data is stored on the hard drive of the instrument computer, transferred to a mirrored storage disk and to a raid 5 server operating on the Partners Healthcare network and backed up nightly via the Partners Healthcare Systems IT backup utility. All systems are CFR21-11 and HIPPA compliant
Description Sample # Group OD 260/280 RNA, ug/ul Actb Ct Chip
1 PregPBS 2.0 0.63 13.8 a
2 PregPBS 2.0 0.647 13.3 j
3 PregPBS 2.1 0.612 13.4 l
4 PregPBS 2.0 0.575 13.8 m
6 PregTiO 2.1 0.585 14.3 n
8 PregTiO 2.1 0.458 13.8 o
9 NormPBS 2.0 0.627 13.3 p
11 NormPBS 2.0 0.714 13.4 b
12 NormPBS 2.1 0.462 13.8 c
13 NormTiO 2.0 0.572 15.6 d
14 NormTiO 2.1 0.598 13.1 e
15 NormTiO 2.1 0.682 13.6 f
16 NormTiO 2.1 0.654 13.6 g
17 PregTiO 2.1 0.586 13.7 h
18 PregTiO 2.0 0.833 12.8 i
20 NormPBS 2.0 0.804 12.5 k
Data processing GCOS/MAS5 and RMA-SAM-ANOVA
 
Submission date Apr 06, 2007
Last update date Aug 28, 2018
Contact name Alexey Fedulov
E-mail(s) afedulov@rics.bwh.harvard.edu
Organization name Harvard Medical School
Department Brigham and Women's Hospital
Lab Fedulov lab
Street address 75 Francis St.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL1261
Series (1)
GSE7475 Inflammatory response to titanium dioxide particles exposure is enhanced during pregnancy
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE value
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 120.788 P 0.000753643
AFFX-BioB-M_at 213.978 P 8.14279e-05
AFFX-BioB-3_at 115.275 P 0.000195116
AFFX-BioC-5_at 389.296 P 5.16732e-05
AFFX-BioC-3_at 480.454 P 4.42873e-05
AFFX-BioDn-5_at 933.273 P 4.42873e-05
AFFX-BioDn-3_at 2166.03 P 6.02111e-05
AFFX-CreX-5_at 4819.83 P 4.42873e-05
AFFX-CreX-3_at 6050.99 P 4.42873e-05
AFFX-DapX-5_at 9.10175 A 0.195266
AFFX-DapX-M_at 3.14903 A 0.760956
AFFX-DapX-3_at 1.96278 A 0.904333
AFFX-LysX-5_at 0.816173 A 0.883887
AFFX-LysX-M_at 5.18264 A 0.617401
AFFX-LysX-3_at 7.04385 M 0.0542134
AFFX-PheX-5_at 0.882973 A 0.834139
AFFX-PheX-M_at 0.772952 A 0.963431
AFFX-PheX-3_at 3.64972 A 0.354453
AFFX-ThrX-5_at 15.4209 A 0.327079
AFFX-ThrX-M_at 5.03313 A 0.559354

Total number of rows: 45101

Table truncated, full table size 1388 Kbytes.




Supplementary file Size Download File type/resource
GSM180975.CHP.gz 243.2 Kb (ftp)(http) CHP
GSM180975.cel.gz 3.8 Mb (ftp)(http) CEL
Processed data provided as supplementary file

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