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Sample GSM181253 Query DataSets for GSM181253
Status Public on Apr 11, 2007
Title cy5:SHR control, biological rep 1r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from untreated SHR rat
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 184+/-13 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -2.080567896 103.0175 639.4737 63 53 1
2 -0.233098362 79.85965 105.3509 62 54 1
3 0.030622929 86.75 102.2 61 53 0
4 -0.240829386 91.22581 122.3387 61 53 0
5 -0.012008876 92.28814 109.9322 62 54 0
6 -0.195121391 96.16949 124.3729 62 53 0
7 -2.510959893 85.20755 629.6415 63 55 0
8 0.282451552 116.069 113.1207 64 55 1
9 0.054321239 84.61538 96.76923 64.5 55 1
10 -0.367951965 134.431 192.9655 65 54 1
11 -0.008739517 94.85965 111.3333 62 53 0
24 -0.009689016 80.53704 97.66667 61 54 0
25 -0.570305133 234.4677 465.0645 63 54 0
26 -0.127988982 91.22034 114.1356 62 53 0
27 0.173226678 119.8519 124.1481 61 54 0
28 -2.510973253 85.10909 667.1091 61 53 0
29 0.149117659 255.6066 243.7705 61 53 0
30 -0.286683881 89.79661 120.6441 63 53 0
31 -0.008583224 105.1228 124.7895 60 54 0
32 0.050061373 81.43103 96.55172 60 53 0

Total number of rows: 21544

Table truncated, full table size 912 Kbytes.




Supplementary file Size Download File type/resource
GSM181253.txt.gz 5.0 Mb (ftp)(http) TXT

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