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Sample GSM181259 Query DataSets for GSM181259
Status Public on Apr 11, 2007
Title cy5:SHR control, biological rep 4r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from untreated SHR rat
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 184+/-13 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -1.735241375 219.1379 620.8793 63 53 0
2 -0.452012427 82.9661 96.05085 63 52 0
3 -0.062557296 115.2909 101.8 63 54 0
4 -0.155289681 145.2712 119.1356 63 55 0
5 0.254887683 186.5082 107.5574 63 55 0
6 -0.216102912 175.0377 136.4906 63 56 0
7 -2.767139967 97.44262 627.623 64 55 0
8 0.036195308 165.5 113.3448 63 54 0
9 -0.057253456 111.2759 98.5 64 53 0
10 -0.276306064 365.2586 222.2241 63 54 0
11 -0.041619517 157.8393 116.2679 64 55 0
24 -0.125408861 118.2727 106.5455 64 55 0
25 -0.284235666 985.0833 543.0167 65 56 0
26 -0.314415438 113.8302 114.8491 65 56 0
27 0.144662489 255.963 132.7593 64 57 0
28 -2.6877742 110.3934 720.4754 63.5 58 0
29 0.403188863 1110.579 279.7719 64 57 0
30 -0.147933055 139.4444 118.6296 65 58 0
31 0.273507921 228.125 119.3214 64 59 0
32 -0.134212434 105.3929 103.125 64 57 0

Total number of rows: 21544

Table truncated, full table size 914 Kbytes.




Supplementary file Size Download File type/resource
GSM181259.txt.gz 5.0 Mb (ftp)(http) TXT

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