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Sample GSM181268 Query DataSets for GSM181268
Status Public on Apr 11, 2007
Title cy5:SHR treated w/ nifedipine, biological rep 2r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from SHR rat treated w/ nifedipine
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 145+/-9 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -2.76952809 198.15 525.4833 62.5 60.5 0
2 -0.152872545 82.73214 108.7143 62 59 0
3 0.235602819 147.4737 129.3684 63 59 0
4 0.034571268 156.6207 141.3966 63 60 0
5 0.389634181 154.0667 128.0333 61.5 60 0
6 -0.139357839 186.3393 164.125 62 61 0
7 -4.050731408 114.7667 557.9667 62 61 1
8 0.165607232 224.0185 162.7222 63 61 0
9 0.241057346 108.9828 116.4828 62 61 0
10 -0.334109904 325.8036 243.0357 62 59 0
11 0.027311925 159.1429 142.5893 61 59 0
24 0.179836577 127.8103 126.5345 63 62 0
25 -0.174039776 1009.018 566.3636 62 62 0
26 -0.274724619 115.75 136.0893 62 62 0
27 0.174823128 306.1667 192.5167 62 62 0
28 -4.254943716 117.8772 643.386 62 66 1
29 0.201036774 719.9216 345.6667 63 64 0
30 -0.003570365 168.807 151.5614 62 63 0
31 0.148645881 230.7627 167.4237 62 62 0
32 0.200370582 102.8246 115.2807 63 62 0

Total number of rows: 21544

Table truncated, full table size 913 Kbytes.




Supplementary file Size Download File type/resource
GSM181268.txt.gz 5.0 Mb (ftp)(http) TXT

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