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Sample GSM181272 Query DataSets for GSM181272
Status Public on Apr 11, 2007
Title cy5:SHR treated w/ nifedipine, biological rep 4r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from SHR rat treated w/ nifedipine
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 145+/-9 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -2.969576336 112.3607 508.1311 62 57.5 1
2 -0.476914784 72.17857 84.08929 61 56 1
3 0.142501916 105.0877 90.50877 61 57 1
4 -0.100212959 124.0893 104.8929 60 57 1
5 0.203333438 117.7586 92.91379 61 57 1
6 -0.321053717 115.2542 110.2203 61 58 1
7 -3.743598413 82.51667 539.8667 61 57 1
8 0.12331037 135.7288 100.2712 62 57 1
9 0.053691329 79.03279 81.63934 61 58 1
10 -0.168362928 321.6833 186.6833 62 60 0
11 0.198183062 102.459 90.95082 62 60 0
24 0.141701052 86.33898 88 61 62 0
25 -0.301452931 817.3333 446.75 62 63 0
26 0.025847418 93.90323 94.90323 63.5 64 0
27 0.205509801 170.3448 112.8103 62 63 0
28 -3.749252187 87.4 605.45 62 62 0
29 0.151804444 440.6557 191.1148 63 63 0
30 -0.154628848 97.10169 100.7458 64 64 0
31 0.318702283 125.2414 97.37931 63 63 0
32 0.227955495 82.15 85.85 62 64 0

Total number of rows: 21544

Table truncated, full table size 912 Kbytes.




Supplementary file Size Download File type/resource
GSM181272.txt.gz 5.0 Mb (ftp)(http) TXT

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