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Sample GSM181276 Query DataSets for GSM181276
Status Public on Apr 11, 2007
Title cy5:SHR treated w/ nifedipine, biological rep 6r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from SHR rat treated w/ nifedipine
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 145+/-9 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -3.289050298 198.7097 515.0968 62 62 0
2 -0.75525864 80.66667 134.2632 61 62 1
3 -0.200946619 121.3621 134.2241 60 61 0
4 -0.679776154 148.9831 161.7797 61 62 0
5 -0.269951438 142.1452 143.129 61 62 0
6 -0.654547025 141.2373 158.1864 60 62 0
7 -4.532203089 96.67241 491.2241 61 62 0
8 0.086848882 144.7458 131.3051 60 61 1
9 -0.112279695 103.6667 127.4386 60 62 0
10 -0.218583475 459.1525 224.9661 62 63 0
11 -0.237348563 183.4211 153.7895 63 63 1
24 -0.377867909 104.1129 137.3548 59.5 64 0
25 -0.297695737 1273.262 474.377 61 64 0
26 -0.669012901 128.5333 155.0333 60 63 0
27 0.054616042 240.4828 157.6724 60 65 0
28 -4.676773055 97.96667 543.6667 59 66 0
29 -0.035658147 566.9091 237.9636 60 66 0
30 -0.463531713 164.8772 160.9474 60 65 0
31 -0.135193718 175.4762 150.3651 60 65 0
32 -0.346903934 96.7619 134.5079 60 65 0

Total number of rows: 21544

Table truncated, full table size 913 Kbytes.




Supplementary file Size Download File type/resource
GSM181276.txt.gz 5.0 Mb (ftp)(http) TXT

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