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Sample GSM181278 Query DataSets for GSM181278
Status Public on Apr 11, 2007
Title cy5:WKY treated w/ angtiotensin, biological rep 1r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from WKY rat treated w/ angiotensin
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 188+/-12 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -3.521535754 170.7581 747.0323 57 49 0
2 -0.383485126 71.61818 109.2182 57 48 0
3 -0.077940169 103.5167 132.8333 57 47 0
4 -0.247778049 113.4821 147.875 57 47 0
5 0.116570374 113.1207 134.2586 57 48 0
6 -0.282651161 125.1207 160.2931 57 48 0
7 -4.814348581 102.7759 732.7241 57 48 1
8 0.099136913 139.6875 153.7917 56 48 0
9 0.092783321 82.5 111.8966 56 48 0
10 -0.340362698 236.1852 249.5 58 48 0
11 -0.02773855 105.6833 133.8 57 48 0
24 -0.284907457 96.89831 135.0678 59.5 50 0
25 -0.452329654 651.4828 604.3793 58 49 0
26 -0.400802946 105.5741 147.6667 57.5 48 0
27 -0.056865669 217.3279 213.3115 57 49 0
28 -5.055782758 108.3115 880.0164 57 49 0
29 0.016899633 480.1207 372.9655 58 50 0
30 -0.361647239 121.4074 162.0556 58 50 0
31 -0.117646806 156.4314 175 59 49 0
32 -0.165835893 89.81633 125.9592 57 49 0

Total number of rows: 21544

Table truncated, full table size 913 Kbytes.




Supplementary file Size Download File type/resource
GSM181278.txt.gz 5.0 Mb (ftp)(http) TXT

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