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Sample GSM181279 Query DataSets for GSM181279
Status Public on Apr 11, 2007
Title cy3:WKY treated w/ angtiotensin, biological rep 2g; cy5:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from WKY rat treated w/ angiotensin
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 188+/-12 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE -(log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed
INV_VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash

Data table
ID_REF VALUE R G Rb Gb FLAGGED INV_VALUE
1 3.34458 219.6 634.3667 65 54 0 -3.344578474
2 -0.140442 117.3929 132.7857 64 53 1 0.140441643
3 -0.460603 142.375 132.7321 65 53 0 0.460602536
4 0.0973877 156.6786 158.4107 63 52 0 -0.09738765
5 -0.205026 155.5714 145.625 64 52 0 0.205026301
6 0.0630805 173.1053 164.9474 63 53 0 -0.063080471
7 4.39411 126.3443 585.3279 64 53 0 -4.394112563
8 -0.219412 230.3571 171.0357 66 52 0 0.219412108
9 -0.256697 153.1897 143.4483 65 53 0 0.25669738
10 -0.178156 374.4561 225.5088 66 53 0 0.178155503
11 -0.303968 170.2 147.3167 64 52 0 0.30396763
24 -0.252366 119.339 130.3729 64 53 0 0.252365757
25 -0.323311 943.8814 400.322 65 53 0 0.323311217
26 -1.27417 406.8654 165.1731 65 54 0 1.27417297
27 -0.330965 210.2545 159.9455 63 52 0 0.330964952
28 4.82435 118.4 655.34 63 52 0 -4.824349803
29 -0.0442308 493.3393 282.8036 64 53 0 0.044230785
30 0.00889327 148.6842 150.4211 65 52 0 -0.008893273
31 -0.248454 174.9833 151.15 64 52 0 0.248454276
32 -0.417165 112.1034 123.6034 62 53 0 0.417165125

Total number of rows: 21544

Table truncated, full table size 1119 Kbytes.




Supplementary file Size Download File type/resource
GSM181279.txt.gz 5.0 Mb (ftp)(http) TXT

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