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Sample GSM181280 Query DataSets for GSM181280
Status Public on Apr 11, 2007
Title cy5:WKY treated w/ angtiotensin, biological rep 2r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from WKY rat treated w/ angiotensin
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 188+/-12 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -4.491764661 97.08475 442.678 60 49 1
2 -0.055618094 80.08333 104.7333 60 48 1
3 0.394736976 87.2069 103.3276 60 48.5 1
4 -0.247524795 105.5926 132.7037 61 48 1
5 0.127685812 117.2712 130.678 60 48 1
6 0.203574773 142.0357 144.7143 61 49 1
7 -5.09320048 86.81667 452.85 61 49 1
8 0.400437553 113.0508 120.2881 60 48 1
9 0.305386544 87.38889 104.7593 60 48 1
10 -0.181131349 211.9833 212.0167 61 48 1
11 0.17641698 125.4655 134.8966 61 49 1
24 0.17412053 89.96491 109.8772 61 49 1
25 -0.124853877 567.4237 474.7458 60 49 1
26 0.064883087 99 120.6 60 49 1
27 0.356399571 133.0847 134.4407 60 49 1
28 -5.139385635 91.41667 511.5667 60 49 0
29 0.27254223 317.8276 247.7241 60 48 1
30 0.110234898 130.4 141.7333 59 49 1
31 0.30995941 124.1071 131.1071 59 49 1
32 0.253856229 88.08333 106.4167 60 48 1

Total number of rows: 21544

Table truncated, full table size 913 Kbytes.




Supplementary file Size Download File type/resource
GSM181280.txt.gz 5.0 Mb (ftp)(http) TXT

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