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Sample GSM181286 Query DataSets for GSM181286
Status Public on Apr 11, 2007
Title cy5:WKY treated w/ angtiotensin, biological rep 5r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from WKY rat treated w/ angiotensin
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 188+/-12 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -4.160518248 114.4068 531.8644 60 51 0
2 -0.036939912 81.42857 109.3929 60 51 0
3 0.223494342 100.1967 114.1803 58 51 0
4 -0.118775319 123.35 132.6667 60 51 0
5 0.192748823 113.3091 120.5818 59 52 0
6 -0.057606277 131.7593 133.6852 60 51 0
7 -4.662979502 93.01786 535.0179 60 52 0
8 0.366387711 166.3621 130.5345 60 52 0
9 0.356536106 100.3443 111.459 59 52 0
10 0.061144491 423 216.434 58.5 52 0
11 0.287612194 158.1228 131.3158 59 52 0
24 0.14458487 94.27119 115.6441 60 54 0
25 0.187784244 1496.033 486.6167 59 55 0
26 -0.097559885 116.0678 133.5085 59 55 0
27 0.361658417 180.9273 137.6909 59 55 0
28 -4.888268584 93.33929 607.9464 58 54 0
29 0.172466507 625.5333 263.5333 58 55 0
30 -0.041119036 139 141.0727 59 56 0
31 0.280396758 150.7333 133.05 60.5 56 0
32 0.269294316 90.35593 112.3559 60.5 56 0

Total number of rows: 21544

Table truncated, full table size 913 Kbytes.




Supplementary file Size Download File type/resource
GSM181286.txt.gz 5.0 Mb (ftp)(http) TXT

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