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Sample GSM181300 Query DataSets for GSM181300
Status Public on Apr 11, 2007
Title cy5:WKY control, biological rep 6r; cy3:pool of 6 WKY control rats
Sample type RNA
 
Channel 1
Source name medial layer of thorassic and abdominal aorta from untreated WKY rat
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy5
Label protocol fluorescent linear amplification kit from Agilent
 
Channel 2
Source name medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
Organism Rattus norvegicus
Characteristics mean systolic blood pressure = 107+/-8 mm HG
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit
Label Cy3
Label protocol fluorescent linear amplification kit from Agilent
 
 
Hybridization protocol 60 C for 17 hours
Scan protocol Agilent DNA Microarray scanner
Description na
Data processing images analyzed with Agilent Feature Extraction v7.1,
 
Submission date Apr 09, 2007
Last update date Apr 10, 2007
Contact name Lynn M Amon
E-mail(s) lynn.amon@cidresearch.org
Organization name Fred Hutchinson Cancer Research Center
Department Division of Public Health Sciences
Lab Computation Biology Program
Street address 1100 Fairview, M1-C108
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
 
Platform ID GPL890
Series (1)
GSE7483 Microarray analysis of aortic tissue from different rat hypertensive models

Data table header descriptions
ID_REF Agilent Feature Extraction feature number
VALUE log2((R-Rb)/(G-Gb)) Loess normalized in LIMMA and scaled normalized across arrays excl SHR_wash
R rMeanSignal
G gMeanSignal
Rb rBGMedianSignal
Gb gBGMedianSignal
FLAGGED feature or background signal was nonuniform or outlier flagged and removed

Data table
ID_REF VALUE R G Rb Gb FLAGGED
1 -4.220714092 112.9333 524.5167 61.5 60 0
2 -0.681228979 72.40984 95.72131 61 58 0
3 0.546506114 107.5091 106.6364 60 58 0
4 0.282198963 104.8727 111.0182 60 58 0
5 0.12342479 93.66038 107.6981 60 59 0
6 0.286516407 111.9286 117.0714 60 60 0
7 -5.49998782 81.07692 514.3269 61 59 1
8 0.753474785 118.6071 109.0714 61 60 0
9 0.539616851 81.15517 89.82759 60 59 0
10 0.04220369 266.5263 211.4561 61 59 0
11 0.437385093 109.7895 111.7193 61 60 0
24 0.268788404 83.89831 98.37288 60 61 0
25 -0.180037087 699.7627 516.7119 60 60 0
26 -0.054149748 92.77358 111.0755 61 60 0
27 0.441099283 127.3621 119.9828 60.5 59 0
28 -5.821981091 79.86792 587.0755 60 61 0
29 0.168884275 361.8 253.85 60 60 0
30 0.329172357 102.1864 110.9153 59 60 0
31 0.51830748 121.5167 115.85 60 59.5 0
32 -0.207252246 76.09836 94.68852 61 59 0

Total number of rows: 21544

Table truncated, full table size 913 Kbytes.




Supplementary file Size Download File type/resource
GSM181300.txt.gz 5.0 Mb (ftp)(http) TXT

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