|
| Status |
Public on Apr 11, 2007 |
| Title |
cy3:SHR treated w/ nifedipine and then washed out, biological rep 5g; cy5:pool of 6 WKY control rats |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
medial layer of thorassic and abdominal aorta from pool of 6 WKY control rats
|
| Organism |
Rattus norvegicus |
| Characteristics |
mean systolic blood pressure = 107+/-8 mm HG
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
|
| Label |
Cy5
|
| Label protocol |
fluorescent linear amplification kit from Agilent
|
| |
|
| Channel 2 |
| Source name |
medial layer of thorassic and abdominal aorta from SHR rat treated w/ nifedipine and then washed out
|
| Organism |
Rattus norvegicus |
| Characteristics |
mean systolic blood pressure = 151+/-4 mm HG
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit
|
| Label |
Cy3
|
| Label protocol |
fluorescent linear amplification kit from Agilent
|
| |
|
| |
| Hybridization protocol |
60 C for 17 hours
|
| Scan protocol |
Agilent DNA Microarray scanner
|
| Description |
na
|
| Data processing |
images analyzed with Agilent Feature Extraction v7.1,
|
| |
|
| Submission date |
Apr 09, 2007 |
| Last update date |
Apr 10, 2007 |
| Contact name |
Lynn M Amon |
| E-mail(s) |
lynn.amon@cidresearch.org
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Division of Public Health Sciences
|
| Lab |
Computation Biology Program
|
| Street address |
1100 Fairview, M1-C108
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-1024 |
| Country |
USA |
| |
|
| Platform ID |
GPL890 |
| Series (1) |
| GSE7483 |
Microarray analysis of aortic tissue from different rat hypertensive models |
|
