|
| Status |
Public on Mar 05, 2004 |
| Title |
LPS and LPS-binding protein treated RAW 264.7 cells 2 hours vs Untreated RAW 264.7 cells 2 hours (set 1) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.7 cells was labeled with Cyanine-3 (green).
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
Total RNA from untreated RAW 264.7 cells was labeled with Cyanine-5 (red).
|
| Organism |
Mus musculus |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.7 cells cultured for 2 hours.
All samples were gathered from mouse RAW 264.7 cells (macrophages). Test total RNA was extracted from untreated RAW 264.4 cells cultured for 2 hours.
|
| |
|
| Submission date |
Mar 03, 2004 |
| Last update date |
May 27, 2005 |
| Contact name |
Sangdun Choi |
| E-mail(s) |
schoi@its.caltech.edu
|
| Phone |
626-395-8732
|
| URL |
http://www.signaling-gateway.org
|
| Organization name |
California Institute of Technology
|
| Department |
Biology
|
| Lab |
Alliance for Cellular Signaling Molecular Biology Laboratory
|
| Street address |
1200 E. California Blvd
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platform ID |
GPL254 |
| Series (2) |
| GSE1098 |
Effect of LPS and LPS-binding protein treatment on RAW 264.4 cells |
| GSE1100 |
Effect of LPS and LPS-binding protein treatment for 2 hours on RAW 264.4 cells |
|
| Data table header descriptions |
| ID_REF |
Feature number which correspond to the order of genes on the array |
| G_PROCESSED |
Processed Signal, after dye normalization and background subtraction, used for computing the log ratio. |
| G_MEAN |
Green Channel Mean Raw Intensity |
| G_MEDIAN |
Green Channel Median Raw Intensity |
| G_SD |
Green Channel Standard Deviation |
| G_BG_MEAN |
Green Channel Mean Background Level |
| G_BG_MEDIAN |
Green Channel Median Background Level |
| G_BG_SD |
Green Channel Background Standard Deviation |
| R_PROCESSED |
Processed Signal, after dye normalization and background subtraction, used for computing the log ratio. |
| R_MEAN |
Red Channel Mean Raw Intensity |
| R_MEDIAN |
Red Channel Median Raw Intensity |
| R_SD |
Red Channel Standard Deviation |
| R_BG_MEAN |
Red Channel Mean Background Level |
| R_BG_MEDIAN |
Red Channel Median Background Level |
| R_BG_SD |
Red Channel Background Standard Deviation |
| RATIO |
Log Ratio (base 10) |
| VALUE |
Processed Ratio (base 2) after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots. |
