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Status |
Public on Jan 11, 2016 |
Title |
Gene expression MLL-AF9 induced leukemic bone marrow ckit-neg MLL-AF9-73-0-ckit-neg |
Sample type |
RNA |
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|
Source name |
leukemic spleen
|
Organism |
Mus musculus |
Characteristics |
dnmt3b overexpression: WT ckit facs: ckit-neg oncogene: MLL-AF9
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (QIAgen Hilden). RNA quality was approved using the Agilent RNA 6000 Nano Kit (Agilent Technologies).
|
Label |
biotin
|
Label protocol |
The samples were labeled according to the Affymetrix GeneChip WT Terminal Labeling protocol (Affymetrix UK Ltd., High Wycombe, UK).
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|
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Hybridization protocol |
Arrays were hybridized and washed according to the manufacturer's specifications (Affymetrix Inc., Santa Clara, CA, USA)
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Scan protocol |
The arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Description |
Gene expression data from MLL-AF9 induced leukemia from bone marrow ckit-neg
|
Data processing |
Expression data was normalized, background corrected and summarized using the RMA algorithm implemented in the Affymetrix Expression Console software Build 1.3.1.187.
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|
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Submission date |
Jul 17, 2015 |
Last update date |
Jan 11, 2016 |
Contact name |
Christian Rohde |
E-mail(s) |
christian.rohde@uni-heidelberg.de
|
Organization name |
Heidelberg University
|
Lab |
Molecular Hematology and Oncology
|
Street address |
Im Neuenheimer Feld 410
|
City |
Heidelberg |
ZIP/Postal code |
69120 |
Country |
Germany |
|
|
Platform ID |
GPL16570 |
Series (2) |
GSE71040 |
Increased DNA Methylation of Dnmt3b-Targets Impairs Leukemogenesis (expression) |
GSE71041 |
Increased DNA Methylation of Dnmt3b-Targets Impairs Leukemogenesis |
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