|
| Status |
Public on Jan 11, 2016 |
| Title |
Gene expression MLL-AF9 induced leukemic bone marrow ckit-neg overexpression Dnmt3b MLL-AF9-83-0-ckit-neg |
| Sample type |
RNA |
| |
|
| Source name |
leukemic spleen
|
| Organism |
Mus musculus |
| Characteristics |
dnmt3b overexpression: Dnmt3b-knockin
ckit facs: ckit-neg
oncogene: MLL-AF9
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (QIAgen Hilden). RNA quality was approved using the Agilent RNA 6000 Nano Kit (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
The samples were labeled according to the Affymetrix GeneChip WT Terminal Labeling protocol (Affymetrix UK Ltd., High Wycombe, UK).
|
| |
|
| Hybridization protocol |
Arrays were hybridized and washed according to the manufacturer's specifications (Affymetrix Inc., Santa Clara, CA, USA)
|
| Scan protocol |
The arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from MLL-AF9 induced leukemia from bone marrow with Dnmt3b overexpression ckit-neg
|
| Data processing |
Expression data was normalized, background corrected and summarized using the RMA algorithm implemented in the Affymetrix Expression Console software Build 1.3.1.187.
|
| |
|
| Submission date |
Jul 17, 2015 |
| Last update date |
Jan 11, 2016 |
| Contact name |
Christian Rohde |
| E-mail(s) |
christian.rohde@uni-heidelberg.de
|
| Organization name |
Heidelberg University
|
| Lab |
Molecular Hematology and Oncology
|
| Street address |
Im Neuenheimer Feld 410
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE71040 |
Increased DNA Methylation of Dnmt3b-Targets Impairs Leukemogenesis (expression) |
| GSE71041 |
Increased DNA Methylation of Dnmt3b-Targets Impairs Leukemogenesis |
|
