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Sample GSM18279 Query DataSets for GSM18279
Status Public on Mar 10, 2004
Title anaerobic_fnr_rep3
Sample type RNA
Source name anaerobic growth of fnr knock-out strain on M9 media with glucose
Organism Escherichia coli
Extracted molecule total RNA
Description The purpose of this study is to investigate the changes of global gene expression in E. coli during an oxygen shift. All cultures were grown under aerobic or anaerobic conditions in M9 minimal media supplemented with glucose. Samples were RNA-stabilized using Qiagen RNAProtect Bacterial Reagent, and total RNA was isolated from exponentially growing cells using a Qiagen RNeasy mini kit (protocols available at The RNA (10 µg) was then used as the template for cDNA synthesis, the product of which was fragmented, labelled, and hybridized to an Affymetrix E. coli Antisense Genome Array, which was washed and scanned to obtain an image. All of these steps were performed according to Affymetrix protocols (available at
Submission date Mar 04, 2004
Last update date Aug 01, 2011
Contact name Jinseong Cheong
Phone (858)534-8758
Organization name University of California at San Diego
Street address
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platform ID GPL199
Series (2)
GSE1107 anaerobic knock-out
GSE1121 changes of global gene expression in E. coli during an oxygen shift

Data table header descriptions
VALUE dChip preprocessed and normalized value
ABS_CALL Affymetrix's judgement call whether a value is good or not

Data table
AFFX-BioB-5_at 1270.6 A
AFFX-BioC-3_at 171.5 A
AFFX-CreX-5_at 1.3 A
AFFX-CreX-3_at 1.6 A
AFFX-DapX-5_at 2.1 A
AFFX-DapX-M_at 0.6 A
AFFX-DapX-3_at 1 A
AFFX-LysX-5_at 0.4 A
AFFX-LysX-M_at 0.8 A
AFFX-LysX-3_at 0.8 A
AFFX-PheX-5_at 1.1 A
AFFX-PheX-M_at 0.7 A
AFFX-PheX-3_at 2.1 A
AFFX-ThrX-5_at 15.2 A
AFFX-ThrX-M_at 1.7 A
AFFX-ThrX-3_at 53.1 A
AFFX-TrpnX-5_at 1.6 A
AFFX-TrpnX-M_at 2.6 A
AFFX-TrpnX-3_at 0.6 A
AFFX-HXB2_5_at 2.5 A

Total number of rows: 7312

Table truncated, full table size 194 Kbytes.

Supplementary file Size Download File type/resource
GSM18279.CEL.gz 2.1 Mb (ftp)(http) CEL

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