|
| Status |
Public on Feb 09, 2017 |
| Title |
Normal rectum mucosa_rep5 |
| Sample type |
RNA |
| |
|
| Source name |
Normal colorectal tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal tissue
gender: Female
age: 55
location: rectum
Stage: NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen tissues using the TRIzol RNA isolation reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s specifications.
|
| Label |
Cy3
|
| Label protocol |
Samples were labeled with Cy3 dye using the miRNA Complete Labeling System & Hyb Kit (Agilent Technologies)
|
| |
|
| Hybridization protocol |
The fluorescent labeled samples were hybridized to the Human miRNA microarray Version 3 from the Sanger database v12.0 (Agilent Technologies)
|
| Scan protocol |
The microarray slides were analyzed with an Agilent Microarray Scanner.
|
| Description |
miRNA expression of normal colorectal tissue
N22
|
| Data processing |
microRNA microarray raw data were normalized with the GeneSpring GX software, version 11.5 (Silicon Genetics, Redwood City, CA, USA). Measured microRNAs were estimated as present if their signals could be detected in at least 50% of the samples within each sample type. Finally, the expression profiles of 96 microRNAs were acquired.
|
| |
|
| Submission date |
Jul 20, 2015 |
| Last update date |
Feb 09, 2017 |
| Contact name |
Kaitai Zhang |
| E-mail(s) |
zhangkt_bingyin@sina.cn
|
| Phone |
86-10-87788409
|
| Organization name |
Peking Union Medical College & Cancer Institute (Hospital), Chinese Academy of Medical Sciences
|
| Department |
Department of Etiology and Carcinogenesis
|
| Lab |
State Key Laboratory of Molecular Oncology
|
| Street address |
NO. 17, Pan Jiayuan South, Chao Yang District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100021 |
| Country |
China |
| |
|
| Platform ID |
GPL18509 |
| Series (1) |
| GSE71130 |
Development of colorectal cancer miRNA expression signatures from expression profiles of embryonic development, precancer progression and cancer samples |
|
